Machka K, Balg H, Smith M, Braveny I
Immun Infekt. 1978 Dec;6(6):249-52.
Various methods for determining the activity of co-trimoxazole against haemophilus influenzae have been compared. The results were cortically influenced by antagonistic substances in the medium. The content of p-aminobenzoeacid and thymidine in the medium should be as low as possible. The antagonistic influences are compensated by lysed horseblood, which contains thymidine-phosphorylase. Haeminchloride and nicotinamide adenine dinucleotide (NAD) are better growth-factors than other poorly defined preparations (Supplement C, Fil-des-Enrichment, Iso-VitaleX). For the determination of the minimal inhibition concentration (MIC) we propose the microtiter technique. Likewise a modified agar diffusion test can be recommended for screening purposes. Of the 143 haemophilus influenzae strains tested against co-trimoxazole 134 (94%) were sensitive.
已比较了多种测定复方新诺明对流感嗜血杆菌活性的方法。结果在很大程度上受培养基中拮抗物质的影响。培养基中对氨基苯甲酸和胸腺嘧啶核苷的含量应尽可能低。溶血马血可补偿拮抗作用，因为其含有胸腺嘧啶磷酸化酶。氯化血红素和烟酰胺腺嘌呤二核苷酸（NAD）比其他定义不明确的制剂（补充剂C、富集培养物、异维生素X）是更好的生长因子。为测定最低抑菌浓度（MIC），我们推荐微量滴定技术。同样，改良的琼脂扩散试验也可用于筛选目的。在143株测试的流感嗜血杆菌中，134株（94%）对复方新诺明敏感。
