Huh W K, Kang S O
Laboratory of Biophysics, Department of Microbiology, College of Natural Sciences, and Research Center for Molecular Microbiology, Seoul National University, Seoul 151-742, Republic of Korea.
J Bacteriol. 1999 Jul;181(13):4098-102. doi: 10.1128/JB.181.13.4098-4102.1999.
The AOX1 gene, which encodes an alternative oxidase, was isolated from the genomic DNA library of Candida albicans. The gene encodes a polypeptide consisting of 379 amino acids with a calculated molecular mass of 43,975 Da. The aox1/aox1 mutant strain did not show cyanide-resistant respiration under normal conditions but could still induce cyanide-resistant respiration when treated with antimycin A. The measurement of respiratory activity and Western blot analysis suggested the presence of another AOX. When C. albicans AOX1 was expressed in alternative oxidase-deficient Saccharomyces cerevisiae, it could confer cyanide-resistant respiration on S. cerevisiae.
编码交替氧化酶的AOX1基因是从白色念珠菌的基因组DNA文库中分离出来的。该基因编码一个由379个氨基酸组成的多肽,计算分子量为43975道尔顿。aox1/aox1突变株在正常条件下不表现出抗氰呼吸,但在用抗霉素A处理时仍能诱导抗氰呼吸。呼吸活性的测定和蛋白质免疫印迹分析表明存在另一种AOX。当白色念珠菌AOX1在缺乏交替氧化酶的酿酒酵母中表达时,它可以赋予酿酒酵母抗氰呼吸能力。