Matés J M, Segura J M, Pérez-Gómez C, Rosado R, Olalla L, Blanca M, Sánchez-Jiménez F M
Department of Molecular Biology and Biochemistry, Sciences Faculty, University of Málaga, Spain.
Blood Cells Mol Dis. 1999 Apr;25(2):103-9. doi: 10.1006/bcmd.1999.0234.
Several diseases have been related to oxidative stress. Recently, antioxidant functions have also been linked to anti-inflammatory properties. Cell defenses against reactive oxygen species include antioxidant enzymes. We studied the enzymatic antioxidant capacity in human blood of both red blood and mononuclear cells from patients suffering from an allergic reaction to pollen or house dust mite. We determined superoxide dismutases (SODs), glutathione peroxidase (GSHPx) and catalase (CAT) activities in each cell type. We also determined the extent of thiobarbituric acid reactive substances (TBARS), in order to study the correlation between the cellular enzymatic activities, the redox status and the disease. In mononuclear cells from allergic patients, SODs and CAT activities were enhanced compared to controls. Conversely, a decrease in GSHPx activity was found. In erythrocytes, higher values for GSHPx and SODs and similar CAT activities were found in allergic patients and controls. Interestingly, CuZnSOD and MnSOD activities were enhanced in the same proportion for both, erythrocytes and mononuclear cells. TBARS were also enhanced in both types of cells. The respective enzymatic imbalances in mononuclear cells and erythrocytes, namely, GSHPx/SOD and CAT/SOD, and their consequences are discussed. To our knowledge, this is the first global study of antioxidant enzymes, including TBARS level determinations, in allergy.
几种疾病都与氧化应激有关。最近，抗氧化功能也与抗炎特性联系在一起。细胞对抗活性氧的防御机制包括抗氧化酶。我们研究了对花粉或屋尘螨过敏的患者红细胞和单核细胞中人类血液的酶促抗氧化能力。我们测定了每种细胞类型中的超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSHPx）和过氧化氢酶（CAT）活性。我们还测定了硫代巴比妥酸反应性物质（TBARS）的含量，以研究细胞酶活性、氧化还原状态与疾病之间的相关性。在过敏患者的单核细胞中，与对照组相比，SOD和CAT活性增强。相反，发现GSHPx活性降低。在红细胞中，过敏患者和对照组的GSHPx和SOD值较高，CAT活性相似。有趣的是，红细胞和单核细胞中CuZnSOD和MnSOD活性的增强比例相同。两种类型的细胞中TBARS也都增加。讨论了单核细胞和红细胞中各自的酶失衡，即GSHPx/SOD和CAT/SOD及其后果。据我们所知，这是首次在过敏领域对包括TBARS水平测定在内的抗氧化酶进行全面研究。
