根瘤菌温和噬菌体16-3位点特异性重组基因int和xis的鉴定
Identification of site-specific recombination genes int and xis of the Rhizobium temperate phage 16-3.
作者信息
Semsey S, Papp I, Buzas Z, Patthy A, Orosz L, Papp P P
机构信息
Institute for Molecular Genetics, Agricultural Biotechnology Center, Gödöllo, H-2100 Hungary.
出版信息
J Bacteriol. 1999 Jul;181(14):4185-92. doi: 10.1128/JB.181.14.4185-4192.1999.
Abstract
Phage 16-3 is a temperate phage of Rhizobium meliloti 41 which integrates its genome with high efficiency into the host chromosome by site-specific recombination through DNA sequences of attB and attP. Here we report the identification of two phage-encoded genes required for recombinations at these sites: int (phage integration) and xis (prophage excision). We concluded that Int protein of phage 16-3 belongs to the integrase family of tyrosine recombinases. Despite similarities to the cognate systems of the lambdoid phages, the 16-3 int xis att system is not active in Escherichia coli, probably due to requirements for host factors that differ in Rhizobium meliloti and E. coli. The application of the 16-3 site-specific recombination system in biotechnology is discussed.
摘要
噬菌体16 - 3是苜蓿根瘤菌41的一种温和噬菌体，它通过attB和attP的DNA序列，通过位点特异性重组将其基因组高效整合到宿主染色体中。在此我们报告了在这些位点进行重组所需的两个噬菌体编码基因的鉴定：int（噬菌体整合）和xis（原噬菌体切除）。我们得出结论，噬菌体16 - 3的Int蛋白属于酪氨酸重组酶的整合酶家族。尽管与λ样噬菌体的同源系统有相似之处，但16 - 3 int xis att系统在大肠杆菌中不活跃，这可能是由于苜蓿根瘤菌和大肠杆菌对宿主因子的需求不同。本文还讨论了16 - 3位点特异性重组系统在生物技术中的应用。
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