Ye Z H, Buranen S L, Lee C Y
Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City 66103.
J Bacteriol. 1990 May;172(5):2568-75. doi: 10.1128/jb.172.5.2568-2575.1990.
The DNA fragment encoding the integrase and excisionase genes involved in site-specific recombination of staphylococcal bacteriophage phi 11 was cloned and sequenced. The int and xis genes and the recombination site, attP, were highly clustered in a 1.7-kilobase DNA fragment with the gene order attP-int-xis. The int and xis genes were transcribed divergently, with the int gene transcribed toward the attp site and the xis gene transcribed away from the attP site. The deduced Int is a basic protein of 348 residues with an estimated molecular weight of 41,357. In contrast, the deduced Xis is an acidic protein containing 66 amino acids with an estimated molecular weight of 7,621. The site-specific recombination system of phi 11 was compared with that of a closely related bacteriophage, L54a.
编码参与葡萄球菌噬菌体φ11位点特异性重组的整合酶和切除酶基因的DNA片段被克隆并测序。int和xis基因以及重组位点attP高度聚集在一个1.7千碱基的DNA片段中，基因顺序为attP-int-xis。int和xis基因以相反方向转录，int基因朝着attp位点转录，xis基因背离attP位点转录。推导的Int是一个由348个残基组成的碱性蛋白，估计分子量为41,357。相比之下，推导的Xis是一个含66个氨基酸的酸性蛋白，估计分子量为7,621。将φ11的位点特异性重组系统与密切相关的噬菌体L54a的重组系统进行了比较。
