Biology Department and Institute of Biochemistry, Carleton University, Ottawa, Ontario K1S 5B6, Canada.
Appl Environ Microbiol. 1988 Sep;54(9):2273-6. doi: 10.1128/aem.54.9.2273-2276.1988.
The bacterial host ranges of the conjugatively self-transmissible IncN group plasmid pCU1 and its mobilizable miniderivative, pCU785, were examined. Species of the gram-negative purple bacteria were chosen for this study. Conjugative mobilization of pCU785 into a wide variety of bacteria was facilitated by the presence of oriT of the broad-host-range plasmid RK2 in pCU785. Although the host range of the IncN tra system of pCU1 is broad, the host range of its replicon is limited. However, the pCU1 replicon can be maintained in Agrobacterium, Bradyrhizobium, and Rhizobium species under conditions that select for plasmid maintenance. It is lost efficiently from these populations on release of selection.
研究了可接合自我转移的 IncN 组质粒 pCU1 及其可移动的小型衍生物 pCU785 的细菌宿主范围。本研究选择了革兰氏阴性紫色细菌的物种。pCU785 中 RK2 广泛宿主质粒 oriT 的存在促进了 pCU785 向各种细菌的接合转移。虽然 pCU1 的 IncN tra 系统的宿主范围很广，但它的复制子的宿主范围是有限的。然而，在选择维持质粒的条件下，pCU1 复制子可以在根瘤菌、慢生根瘤菌和根瘤菌中维持。当选择压力释放时，它会从这些群体中有效地丢失。
