Han Z S, Ip Y T
Program in Molecular Medicine, Department of Biochemistry and Molecular Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA.
J Biol Chem. 1999 Jul 23;274(30):21355-61. doi: 10.1074/jbc.274.30.21355.
NF-kappaB/Rel family proteins regulate genes that are critical for many cellular processes including apoptosis, inflammation, immune response, and development. NF-kappaB/Rel proteins function as homodimers or heterodimers, which recognize specific DNA sequences within target promoters. We examined the activity of different Drosophila Rel-related proteins in modulating Drosophila immunity genes by expressing the Rel proteins in stably transfected cell lines. We also compared how different combinations of these transcriptional regulators control the activity of various immunity genes. The results show that Rel proteins are directly involved in regulating the Drosophila antimicrobial response. Furthermore, the drosomycin and defensin expression is best induced by the Relish/Dif and the Relish/Dorsal heterodimers, respectively, whereas the attacin activity can be efficiently up-regulated by the Relish homodimer and heterodimers. These results illustrate how the formation of Rel protein dimers differentially regulate target gene expression.
核因子-κB/Rel家族蛋白调控着许多细胞过程中至关重要的基因，这些过程包括细胞凋亡、炎症、免疫反应和发育。核因子-κB/Rel蛋白以同型二聚体或异型二聚体的形式发挥作用，它们可识别靶启动子内的特定DNA序列。我们通过在稳定转染的细胞系中表达Rel蛋白，研究了不同果蝇Rel相关蛋白在调控果蝇免疫基因方面的活性。我们还比较了这些转录调节因子的不同组合如何控制各种免疫基因的活性。结果表明，Rel蛋白直接参与调控果蝇的抗菌反应。此外，分别由Relish/Dif和Relish/Dorsal异型二聚体可最佳地诱导果蝇抗菌肽和防御素的表达，而Relish同型二聚体和异型二聚体可有效上调攻击素的活性。这些结果说明了Rel蛋白二聚体的形成如何差异调节靶基因的表达。
