Lazarova G, Tashiro H
Institute of Microbiology, Bulgarian Academy of Sciences, Acad. G. Bontchev, Sofia.
Microbios. 1995;82(332):187-96.
The effect of polyenic antibiotic amphotericin B on photodynamically induced cell damage was investigated using Kluyveromyces fragilis. The photosensitizers applied are known to act via cell membrane damage (rose bengal and toluidine blue) or via DNA modification causing genotoxic effects (8-methoxypsoralen). Methylene blue was shown to cause membrane damage comparable with the effect of rose bengal and toluidine blue. Under conditions of photodynamic damage a pronounced protective effect of the antibiotic was evident in increased cell survival with all of the photosensitizers tested. Mitochondrial activity indicated a tendency of the antibiotic to protect the cells. The protective role of amphotericin B is discussed in the light of possible implications for photodynamic therapy of microbial infections.
使用脆壁克鲁维酵母研究了多烯抗生素两性霉素B对光动力诱导的细胞损伤的影响。所应用的光敏剂已知通过细胞膜损伤起作用（孟加拉玫瑰红和甲苯胺蓝）或通过引起基因毒性作用的DNA修饰（8-甲氧基补骨脂素）起作用。已表明亚甲蓝引起的膜损伤与孟加拉玫瑰红和甲苯胺蓝的作用相当。在光动力损伤条件下，用所有测试的光敏剂处理后，抗生素具有明显的保护作用，细胞存活率增加。线粒体活性表明抗生素有保护细胞的趋势。根据其对微生物感染光动力治疗的可能影响，讨论了两性霉素B的保护作用。
