Tsai T H, Chen Y F, Chen I F, Chen C F
Department of Pharmacology, National Research Institute of Chinese Medicine, Taipei, Taiwan.
J Chromatogr B Biomed Sci Appl. 1999 Jun 11;729(1-2):119-25. doi: 10.1016/s0378-4347(99)00133-4.
To monitor the levels of caffeic acid in rat blood, an on-line microdialysis system coupled with liquid chromatography was developed. The microdialysis probe was inserted into the jugular vein/right atrium of male Sprague-Dawley rats. Caffeic acid (100 mg/kg, i.v.) was then administered via the femoral vein. Dialysates were automatically injected onto a liquid chromatographic system via an on-line injector. Samples were eluted with a mobile phase containing methanol-100 mM monosodium phosphoric acid (35:65, v/v, pH 2.5). The UV detector wavelength was set at 320 nm. The detection limit of caffeic acid was 20 ng/ml. The in vivo recoveries of the microdialysis probe for caffeic acid at 0.5 and 1 microg/ml were 48.34+/-2.68 and 47.64+/-3.43%, respectively (n=6). Intra- and inter-assay accuracy and precision of the analyses were < or = 10% in the range of 0.05 to 10 microg/ml. Pharmacokinetics analysis of results obtained using such a microdialysis-chromatographic method indicated that unbound caffeic acid in the rat fitted best to a biexponential decay model.
为监测大鼠血液中咖啡酸的水平，开发了一种与液相色谱联用的在线微透析系统。将微透析探针插入雄性Sprague-Dawley大鼠的颈静脉/右心房。然后通过股静脉给予咖啡酸（100 mg/kg，静脉注射）。透析液通过在线进样器自动注入液相色谱系统。样品用含有甲醇-100 mM磷酸二氢钠（35:65，v/v，pH 2.5）的流动相洗脱。紫外检测器波长设定为320 nm。咖啡酸的检测限为20 ng/ml。微透析探针在0.5和1 μg/ml时对咖啡酸的体内回收率分别为48.34±2.68%和47.64±3.43%（n = 6）。在0.05至10 μg/ml范围内，分析的批内和批间准确度和精密度≤10%。使用这种微透析-色谱方法获得的结果的药代动力学分析表明，大鼠体内游离咖啡酸最符合双指数衰减模型。
