在线微透析和微径液相色谱法测定大鼠血液中游离头孢美唑：一项药代动力学研究。

Determination of unbound cefmetazole in rat blood by on-line microdialysis and microbore liquid chromatography: a pharmacokinetic study.

作者信息

Tsai T R, Cheng F C, Hung L C, Chen C F, Tsai T H

机构信息

School of Pharmacy, Kaohsiung Medical University, Taiwan.

出版信息

J Chromatogr B Biomed Sci Appl. 1999 Dec 24;736(1-2):129-34. doi: 10.1016/s0378-4347(99)00451-x.

DOI:10.1016/s0378-4347(99)00451-x

PMID:10676992

Abstract

A specific and sensitive microbore liquid chromatographic method for the determination of unbound cefmetazole in rat blood was developed. A microdialysis probe was inserted into the jugular vein/right atrium of a Sprague-Dawley rat. Cefmetazole (10 mg/kg, i.v.) was then administered via the femoral vein. Dialysates were automatically injected into a liquid chromatographic system via an on-line injector. Isocratic elution of cefmetazole was achieved by LC-UV within 10 min. Intra- and inter-assay accuracy and precision of the assay were < or = 10%. The detection limit of cefmetazole was 20 ng/ml. Pharmacokinetic analysis of results indicated that unbound cefmetazole levels in rats best fit a biexponential decay model.

摘要

建立了一种用于测定大鼠血液中游离头孢美唑的特异性灵敏微径液相色谱法。将微透析探针插入Sprague-Dawley大鼠的颈静脉/右心房。然后经股静脉给予头孢美唑（10mg/kg，静脉注射）。透析液通过在线进样器自动注入液相色谱系统。通过LC-UV在10分钟内实现了头孢美唑的等度洗脱。该测定法的批内和批间准确度和精密度均≤10%。头孢美唑的检测限为20ng/ml。结果的药代动力学分析表明，大鼠体内游离头孢美唑水平最符合双指数衰减模型。