Tsai T H, Cheng F C, Hung L C, Chen C F
Department of Pharmacology, National Research Institute of Chinese Medicine, National Yang-Ming University, Taipei, Taiwan.
J Chromatogr B Biomed Sci Appl. 1998 Dec 11;720(1-2):165-9. doi: 10.1016/s0378-4347(98)00435-6.
On-line microdialysis coupled with microbore liquid chromatography was used to investigate the pharmacokinetics of chloramphenicol and its glucuronide in rat blood. A microdialysis probe was inserted into a jugular vein of male Sprague-Dawley rats. Chloramphenicol succinate (20 mg/kg, intravenously) was then administered via a femoral vein. Dialysates were automatically injected onto a LC system, via an on-line injector. Samples were eluted with a mobile phase containing acetonitrile-10 mM monochloroacetic acid (30:70, v/v, pH 3.0). The UV detector wavelength was set at 278 nm. The limit of quantitation for chloramphenicol was 10 ng/ml. The in vitro recoveries of chloramphenicol and chloramphenicol glucuronide at 500 ng/ml were 32.2+/-0.3% and 11.4+/-0.7%, respectively (n = 6). Intra- and inter-assay accuracy and precision of the analyses were < or =10% in the range of 0.01 to 5.0 microg/ml.
采用在线微透析与微径液相色谱联用技术研究大鼠血液中氯霉素及其葡糖醛酸苷的药代动力学。将微透析探针插入雄性Sprague-Dawley大鼠的颈静脉。然后经股静脉给予琥珀氯霉素（20mg/kg，静脉注射）。透析液通过在线进样器自动进样到液相色谱系统中。样品用含乙腈-10mM一氯乙酸（30:70，v/v，pH 3.0）的流动相洗脱。紫外检测器波长设定为278nm。氯霉素的定量限为10ng/ml。氯霉素和氯霉素葡糖醛酸苷在500ng/ml时的体外回收率分别为32.2±0.3%和11.4±0.7%（n = 6）。在0.01至5.0μg/ml范围内，分析的批内和批间准确度和精密度均≤10%。
