Tsai T H, Kao H Y, Chen C F
National Research Institute of Chinese Medicine, 155-1 Li-Nong Street Section 2, Taipei 112, Taiwan.
Biomed Chromatogr. 2001 Apr;15(2):79-82. doi: 10.1002/bmc.40.
A technique involving rapid sampling of cephaloridine in rat blood was achieved using a combination of microdialysis and sensitive microbore liquid chromatography. A microdialysis probe was inserted into the jugular vein/right atrium of a Sprague-Dawley rat. Then after a real-time collection of the analyte by microdialysis, the dialysate was automatically injected into a liquid chromatographic system via an on-line injector. Following a 2 h stabilization period after the surgical procedure, cephaloridine (20 mg/kg, i.v.) was then administered via the femoral vein. Isocratic elution of cephaloridine was carried out with a mobile phase containing methanol-20 mM monosodium phosphate (25:75, v/v, pH 5.5), and the flow rate of the mobile phase was 0.05 mL/min within 10 min. Intra- and inter-assay accuracy and precision of the assay were each less than 10%. The in vivo recovery of the cephaloridine from the microdialysate was 49.7 +/- 8.0% and 42.4 +/- 8.4% for 0.5 and 1 microg/mL standards (n = 6), respectively. Based on the pharmacokinetic analysis, the elimination half-life was 32.2 +/- 8.6 min by cephaloridine administration (20 mg/kg, i.v., n = 6).
采用微透析和灵敏的微径液相色谱联用技术，实现了大鼠血液中头孢菌素的快速采样。将微透析探针插入Sprague-Dawley大鼠的颈静脉/右心房。然后通过微透析实时收集分析物后，透析液通过在线进样器自动注入液相色谱系统。手术操作后经过2小时的稳定期，随后经股静脉给予头孢菌素（20mg/kg，静脉注射）。用含有甲醇-20mM磷酸二氢钠（25:75，v/v，pH 5.5）的流动相进行头孢菌素的等度洗脱，流动相流速在10分钟内为0.05mL/min。该测定的批内和批间准确度和精密度均小于10%。对于0.5和1μg/mL的标准品（n = 6），从微透析液中头孢菌素的体内回收率分别为49.7±8.0%和42.4±8.4%。基于药代动力学分析，给予头孢菌素（20mg/kg，静脉注射，n = 6）后的消除半衰期为32.2±8.6分钟。
