Mafra R A, Figueiredo S G, Diniz C R, Cordeiro M N, Cruz J D, De Lima M E
Departamento de Fisiologia e Biofísica, ICB, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, 31270-901, Belo Horizonte-MG, Brazil.
Brain Res. 1999 Jun 12;831(1-2):297-300. doi: 10.1016/s0006-8993(99)01472-9.
We report the characterization of a new class of glutamate uptake inhibitors isolated from Phoneutria nigriventer venom. Glutamate transport activity was assayed in rat cerebrocortical synaptosomes by using [(3)H]-L-glutamate. PhTx4 inhibited glutamate uptake in a dose dependent manner. The IC(50) value obtained was 2.35+/-0.9 microg/ml which is in the observed range reported for glutamate uptake blockers. Tx4-7, one of PhTx4 toxins, showed the strongest inhibitory activity (50.3+/-0.69%, n=3).
我们报告了从黑腹舞蛛毒液中分离出的一类新型谷氨酸摄取抑制剂的特性。通过使用[³H]-L-谷氨酸在大鼠大脑皮质突触体中测定谷氨酸转运活性。PhTx4以剂量依赖性方式抑制谷氨酸摄取。获得的IC50值为2.35±0.9微克/毫升，这在报道的谷氨酸摄取阻滞剂的观察范围内。PhTx4毒素之一的Tx4-7表现出最强的抑制活性（50.3±0.69%，n = 3）。
