Lange C, Cardoso M, Senczek D, Schwarz S
Institut für Tïerzucht und Tïerverhalten der Bundesforschungsanstalt für Landwirtschaft Braunschweig (FAL), Celle, Germany.
Vet Microbiol. 1999 Jun 15;67(2):127-41. doi: 10.1016/s0378-1135(99)00031-0.
Sixty-six isolates of Staphylococcus aureus obtained from milk samples of dairy cows suffering from subclinical mastitis in southern Brazil were analysed by five different molecular typing methods. These included the analysis of plasmid profiles, the analysis of coagulase (coa) gene polymorphisms by PCR amplification of the 3' terminal region of the coa gene, the PCR-based detection of polymorphisms in the X region of the protein A gene (spa), the PCR-directed analysis of variations in the spacer region between 16S and 23S rRNA, and the comparison of pulsed-field gel electrophoretically separated genomic SmaI fragment patterns. The molecular typing methods were supplemented with the biochemical characterization of the isolates and the determination of their in-vitro susceptibility to 14 different antibiotics. All genotypic and phenotypic typing methods were analyzed for their ability to discriminate between the isolates. Macrorestriction analysis proved to be the most discriminatory single method (D = 0.96) followed by rRNA spacer typing (D = 0.85), coa PCR (D = 0.82), and spa PCR (D = 0.80).
采用五种不同的分子分型方法，对从巴西南部患有亚临床乳腺炎的奶牛乳汁样本中分离出的66株金黄色葡萄球菌进行了分析。这些方法包括质粒图谱分析、通过对coa基因3'末端区域进行PCR扩增来分析凝固酶（coa）基因多态性、基于PCR检测蛋白A基因（spa）X区域的多态性、PCR定向分析16S和23S rRNA之间间隔区的变异，以及比较脉冲场凝胶电泳分离的基因组SmaI片段模式。分子分型方法还辅以分离株的生化特性鉴定及其对14种不同抗生素的体外敏感性测定。分析了所有基因型和表型分型方法区分分离株的能力。宏观限制性分析被证明是最具鉴别力的单一方法（D = 0.96），其次是rRNA间隔区分型（D = 0.85）、coa PCR（D = 0.82）和spa PCR（D = 0.80）。
