Spagnuolo M, Crecchio C, Pizzigallo M D, Ruggiero P
Istituto di Chimica Agraria, Università degli Studi di Bari, Via Amendola 165/a, 70126 Bari, Italy.
Biotechnol Bioeng. 1999 Sep 20;64(6):685-91. doi: 10.1002/(sici)1097-0290(19990920)64:6<685::aid-bit7>3.0.co;2-e.
Incubation of beet pulp with two arabinases (alpha-L-arabinofuranosidase and endo-arabinase), used singularly or in combination at different units of activity per gram of beet pulp, caused the hydrolysis of arabinan, which produced a hydrolyzate consisting mainly of arabinose. Pectin and a residue enriched with cellulose were subsequently separated from the incubation mixture. The best enzymatic hydrolysis results were obtained when 100 U/g of beet pulp of each enzyme worked synergistically with yields of 100% arabinose and 91.7% pectin. These yields were higher than those obtained with traditional chemical hydrolysis. The pectin fraction showed a low content of neutral sugar content and the cellulose residue contained only a small amount of pentoses. Semicontinuous hydrolysis with enzyme recycling in an ultrafiltration unit was also carried out to separate arabinose, pectin, and cellulose from beet pulp in 7 cycles of hydrolysis followed by ultrafiltration. The yields of separation were similar to those obtained in batch experiments, with an enzyme consumption reduced by 3.5 times and some significant advantages over batch processes.
用两种阿拉伯糖苷酶（α-L-阿拉伯呋喃糖苷酶和内切阿拉伯糖苷酶）对甜菜粕进行孵育，这两种酶单独使用或以每克甜菜粕不同活性单位组合使用，均可导致阿拉伯聚糖水解，产生主要由阿拉伯糖组成的水解产物。随后从孵育混合物中分离出果胶和富含纤维素的残余物。当每种酶以100 U/g甜菜粕协同作用时，酶促水解效果最佳，阿拉伯糖产率为100%，果胶产率为91.7%。这些产率高于传统化学水解所获得的产率。果胶部分的中性糖含量较低，纤维素残余物仅含有少量戊糖。还进行了在超滤装置中酶循环的半连续水解，以在7次水解和超滤循环中从甜菜粕中分离出阿拉伯糖、果胶和纤维素。分离产率与分批实验获得的产率相似，酶消耗减少了3.5倍，且比分批工艺具有一些显著优势。
