The Advanced Centre for Biochemical Engineering, Department of Biochemical Engineering, Bernard Katz Building, University College London, Gower Street, London WC1E 6BT, UK.
Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath BA2 7AY, UK.
Bioresour Technol. 2018 Dec;269:195-202. doi: 10.1016/j.biortech.2018.08.069. Epub 2018 Aug 22.
Sugar beet pulp (SBP) fractionated by steam explosion, released sugar beet pectin (SB-pectin) which was selectively hydrolysed using a novel α-l-arabinofuranosidase (AF), yielding monomeric l-arabinose (Ara) and a galacturonic acid rich backbone (GABB). AF was immobilised on an epoxy-functionalised resin with 70% overall immobilisation yield. Pretreatment of SB-pectin, to remove coloured compounds, improved the stability of the immobilised AF, allowing its reutilisation for up to 10 reaction cycles in a stirred tank reactor. Continuous hydrolysis of SB-pectin was subsequently performed using a packed bed reactor (PBR) with immobilised AF. Reactor performance was evaluated using a Design of Experiment approach. Pretreated SB-pectin hydrolysis was run for 7 consecutive days maintaining 73% of PBR performance. Continuous separation of Ara from GABB was achieved by tangential flow ultrafiltration with 92% Ara recovery. These results demonstrate the feasibility of establishing a continuous bioprocess to obtain Ara from the inexpensive SBP biomass.
用蒸汽爆破对糖甜菜渣进行分级，释放出糖甜菜果胶（SB-pectin），然后使用新型α-L-阿拉伯呋喃糖苷酶（AF）选择性水解，生成单糖 L-阿拉伯糖（Ara）和富含半乳糖醛酸的主链（GABB）。AF 被固定在一种环氧功能化树脂上，总固定化收率为 70%。对 SB-pectin 进行预处理以去除有色化合物，提高了固定化 AF 的稳定性，使其在搅拌釜反应器中重复使用多达 10 次反应循环。随后使用填充床反应器（PBR）和固定化 AF 连续水解 SB-pectin。使用实验设计方法评估了反应器的性能。预处理后的 SB-pectin 水解连续进行了 7 天，保持了 PBR 性能的 73%。通过切向流超滤连续分离 Ara 和 GABB，Ara 的回收率为 92%。这些结果表明，从廉价的 SBP 生物质中获得 Ara 的连续生物过程是可行的。
