Santos W R, Paraguai de Souza E, Palatnik M, Palatnik de Sousa C B
Instituto de Microbiologia, Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro (UFRJ), CCS, Cidade Universitária, Brazil.
Vaccine. 1999 Jun 4;17(20-21):2554-61. doi: 10.1016/s0264-410x(99)00058-4.
The FML antigen of Leishmania donovani in combination with saponin, aluminum hydroxide (Al(OH)3) and Freund's incomplete adjuvant (FIA) was used in vaccines tested in an outbred murine model of visceral leishmaniasis, either through intraperitoneal or subcutaneous routes. The humoral response was significantly higher in the groups treated with FML + saponin or FML + Al(OH)3 than in controls, both before and after the infection. Animals immunized by the i.p. route developed higher antibody titres. A significant and specific reduction of parasitic load in relation to saline (85%, p < 0.01) and saponin (p < 0.025) controls, was seen in animals treated with FML + saponin by the i.p. Coincidentally with this reduction, an increase in antibodies of the IgG2a subtype was detected only in animals treated with FML + saponin i.p. A reduction of 88% in parasitic load was achieved by the combination of FML + Al(OH)3 (s.c.), but the Al(OH)3 treatment itself accounted for 68% of this protection. In our conditions, vaccination with FML + saponin i.p. was superior to other treatments and had no toxic effect due to saponin.
杜氏利什曼原虫的FML抗原与皂苷、氢氧化铝（Al(OH)3）和弗氏不完全佐剂（FIA）联合，通过腹腔内或皮下途径，用于在内脏利什曼病远交系小鼠模型中测试的疫苗。在感染前后，用FML +皂苷或FML + Al(OH)3处理的组的体液反应均显著高于对照组。通过腹腔内途径免疫的动物产生了更高的抗体滴度。与盐水（85%，p < 0.01）和皂苷（p < 0.025）对照组相比，腹腔内注射FML +皂苷处理的动物的寄生虫负荷显著且特异性降低。与此降低同时，仅在腹腔内注射FML +皂苷处理的动物中检测到IgG2a亚型抗体增加。FML + Al(OH)3（皮下）联合使用使寄生虫负荷降低了88%，但Al(OH)3处理本身占这种保护作用的68%。在我们的条件下，腹腔内注射FML +皂苷进行疫苗接种优于其他处理，并且皂苷没有毒性作用。
