相关链霉菌质粒pSB24.2对淡青链霉菌质粒pIJ101接合功能的互补作用。
Complementation of conjugation functions of Streptomyces lividans plasmid pIJ101 by the related Streptomyces plasmid pSB24.2.
作者信息
Pettis G S, Prakash S
机构信息
Department of Biological Sciences, Louisiana State University, Baton Rouge, Louisiana 70803, USA.
出版信息
J Bacteriol. 1999 Aug;181(15):4680-5. doi: 10.1128/JB.181.15.4680-4685.1999.
Abstract
A database search revealed extensive sequence similarity between Streptomyces lividans plasmid pIJ101 and Streptomyces plasmid pSB24. 2, which is a deletion derivative of Streptomyces cyanogenus plasmid pSB24.1. The high degree of relatedness between the two plasmids allowed the construction of a genetic map of pSB24.2, consisting of putative transfer and replication loci. Two pSB24.2 loci, namely, the cis-acting locus for transfer (clt) and the transfer-associated korB gene, were shown to be capable of complementing the pIJ101 clt and korB functions, respectively, a result that is consistent with the notion that pIJ101 and the parental plasmid pSB24.1 encode highly similar, if not identical, conjugation systems.
摘要
数据库搜索显示，淡青链霉菌质粒pIJ101与链霉菌质粒pSB24.2之间存在广泛的序列相似性，pSB24.2是产氰链霉菌质粒pSB24.1的缺失衍生物。这两种质粒之间的高度相关性使得构建了pSB24.2的遗传图谱，该图谱由推定的转移和复制位点组成。两个pSB24.2位点，即转移顺式作用位点（clt）和与转移相关的korB基因，分别被证明能够互补pIJ101的clt和korB功能，这一结果与pIJ101和亲本质粒pSB24.1编码高度相似（如果不是相同）的接合系统这一观点一致。
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