Servín-González L
Departamento de Biología Molecular, Universidad Nacional Autónoma de México, Ciudad Universitaria, D.F., México.
Plasmid. 1993 Sep;30(2):131-40. doi: 10.1006/plas.1993.1040.
The essential replication region of the Streptomyces phaeochromogenes plasmid pJV1 was sequenced and compared to the equivalent region of the well-characterized Streptomyces plasmid pIJ101. The sequence revealed a similar organization in both plasmids, including a conserved region within both plasmid origins, which is just upstream of a gene encoding the replication protein (Rep). The Rep proteins of pJV1 and pIJ101 are very similar, and both have a conserved motif, also present in Rep proteins of single-stranded DNA plasmids of the low G + C gram+ bacteria that includes a tyrosine residue presumably involved in creating the single-stranded nick that initiates the replication event. A co-integration experiment between these two replicons led to the formation of a novel replicon with a hybrid origin and allowed the identification of the nick site where replication starts, also showing that the replication functions of both plasmids are related.
对产色链霉菌质粒pJV1的必需复制区域进行了测序，并与特征明确的链霉菌质粒pIJ101的等效区域进行了比较。序列分析表明，两种质粒具有相似的组织架构，包括两个质粒复制起点内的一个保守区域，该区域位于编码复制蛋白（Rep）的基因上游。pJV1和pIJ101的Rep蛋白非常相似，并且都有一个保守基序，该基序也存在于低G + C革兰氏阳性菌的单链DNA质粒的Rep蛋白中，其中包括一个酪氨酸残基，可能参与产生引发复制事件的单链切口。这两个复制子之间的共整合实验导致形成了一个具有杂合起源的新型复制子，并确定了复制起始的切口位点，这也表明两种质粒的复制功能是相关的。
