嗜铬链霉菌质粒pJV1中一个新的clt基因座的鉴定及特性
Identification and properties of a novel clt locus in the Streptomyces phaeochromogenes plasmid pJV1.
作者信息
Servín-González L
机构信息
Departamento de Biología Molecular, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, Mexico.
出版信息
J Bacteriol. 1996 Jul;178(14):4323-6. doi: 10.1128/jb.178.14.4323-4326.1996.
Abstract
A novel clt locus required for efficient transfer of the Streptomyces phaeochromogenes plasmid pJV1 was identified and mapped. The clt region was functional in both orientations, and its absence caused a severe reduction in plasmid transfer. Chromosome mobilization, on the other hand, was not affected by absence of the clt locus. The clt region showed structural, but not sequence, similarity to transfer origins of gram-negative plasmids.
摘要
一个对产色链霉菌质粒pJV1高效转移所必需的新型clt基因座被鉴定并定位。clt区域在两个方向上均有功能，其缺失导致质粒转移严重减少。另一方面，染色体迁移不受clt基因座缺失的影响。clt区域与革兰氏阴性菌质粒的转移起始点在结构上而非序列上具有相似性。
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