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本文引用的文献

1
Derivatives of pUC18 that have BglII sites flanking a modified multiple cloning site and that retain the ability to identify recombinant clones by visual screening of Escherichia coli colonies.pUC18的衍生物，其在修饰的多克隆位点两侧具有BglII位点，并保留了通过对大肠杆菌菌落进行视觉筛选来鉴定重组克隆的能力。
Gene. 1993 Feb 14;124(1):133-4. doi: 10.1016/0378-1119(93)90774-w.
2
Relationship between the replication functions of Streptomyces plasmids pJV1 and pIJ101.链霉菌质粒pJV1和pIJ101的复制功能之间的关系
Plasmid. 1993 Sep;30(2):131-40. doi: 10.1006/plas.1993.1040.
3
Complete nucleotide sequence of the Streptomyces nigrifaciens plasmid, pSN22: genetic organization and correlation with genetic properties.黑色链霉菌质粒pSN22的完整核苷酸序列：遗传组织及其与遗传特性的相关性
Plasmid. 1994 Jul;32(1):55-69. doi: 10.1006/plas.1994.1044.
4
Transfer of the plJ101 plasmid in Streptomyces lividans requires a cis-acting function dispensable for chromosomal gene transfer.在淡紫链霉菌中，plJ101质粒的转移需要一种顺式作用功能，而这种功能对于染色体基因转移是可有可无的。
Mol Microbiol. 1994 Sep;13(6):955-64. doi: 10.1111/j.1365-2958.1994.tb00487.x.
5
Specific binding of the NikA protein to one arm of 17-base-pair inverted repeat sequences within the oriT region of plasmid R64.NikA蛋白与质粒R64 oriT区域内17个碱基对反向重复序列的一个臂的特异性结合。
J Bacteriol. 1995 Jan;177(1):46-51. doi: 10.1128/jb.177.1.46-51.1995.
6
Streptococcal plasmid pIP501 has a functional oriT site.链球菌质粒pIP501有一个功能性的转移起始位点。
J Bacteriol. 1995 Aug;177(15):4199-206. doi: 10.1128/jb.177.15.4199-4206.1995.
7
A functional origin of transfer (oriT) on the conjugative transposon Tn916.接合转座子Tn916上转移功能起始位点（oriT）
J Bacteriol. 1995 Nov;177(22):6644-51. doi: 10.1128/jb.177.22.6644-6651.1995.
8
Plasmids, recombination and chromosome mapping in Streptomyces lividans 66.变铅青链霉菌66中的质粒、重组及染色体图谱分析
J Gen Microbiol. 1983 Jul;129(7):2257-69. doi: 10.1099/00221287-129-7-2257.
9
Cloning and expression of the tyrosinase gene from Streptomyces antibioticus in Streptomyces lividans.来自抗生链霉菌的酪氨酸酶基因在变铅青链霉菌中的克隆与表达。
J Gen Microbiol. 1983 Sep;129(9):2703-14. doi: 10.1099/00221287-129-9-2703.
10
Properties of in vitro recombinant derivatives of pJV1, a multi-copy plasmid from Streptomyces phaeochromogenes.来自产色链霉菌的多拷贝质粒pJV1的体外重组衍生物的特性
J Gen Microbiol. 1986 Aug;132(8):2071-8. doi: 10.1099/00221287-132-8-2071.

嗜铬链霉菌质粒pJV1中一个新的clt基因座的鉴定及特性

Identification and properties of a novel clt locus in the Streptomyces phaeochromogenes plasmid pJV1.

作者信息

Servín-González L

机构信息

Departamento de Biología Molecular, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, Mexico.

出版信息

J Bacteriol. 1996 Jul;178(14):4323-6. doi: 10.1128/jb.178.14.4323-4326.1996.

DOI:10.1128/jb.178.14.4323-4326.1996
PMID:8763967
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC178196/
Abstract

A novel clt locus required for efficient transfer of the Streptomyces phaeochromogenes plasmid pJV1 was identified and mapped. The clt region was functional in both orientations, and its absence caused a severe reduction in plasmid transfer. Chromosome mobilization, on the other hand, was not affected by absence of the clt locus. The clt region showed structural, but not sequence, similarity to transfer origins of gram-negative plasmids.

摘要

一个对产色链霉菌质粒pJV1高效转移所必需的新型clt基因座被鉴定并定位。clt区域在两个方向上均有功能，其缺失导致质粒转移严重减少。另一方面，染色体迁移不受clt基因座缺失的影响。clt区域与革兰氏阴性菌质粒的转移起始点在结构上而非序列上具有相似性。