Coulter J B, Phinizy J L, Hunnicutt R E, Baumgart M A, Hise E M, Marr L K
Departments of Pathology and Ophthalmology, Scott & White Clinic and Memorial Hospital, Scott, Sherwood and Brindley Foundation, Texas A & M University Health Science Center, College of Medicine, Temple, TX 76508, USA.
Ophthalmic Res. 1999;31(5):337-45. doi: 10.1159/000055556.
To determine if measurement of lens protein in serum is a feasible means to gain information on the physiologic status of the lens in human subjects.
The gamma-crystallin concentration was measured by a sandwich radioimmunoassay in the sera of 280 subjects aged 25-94 years. Medical records were reviewed for diagnoses of cataract and aphakia.
There was no effect of age or sex on the serum gamma-crystallin concentration. There were 57 subjects with cataract and 27 with aphakia. gamma-Crystallin was higher in all cataract groups and lower in aphakia. The mean gamma-crystallin concentrations for selected subject groups were as follows: clear lens 301 pg/ml; pure nuclear cataract 344 pg/ml; pure cortical cataract 439 pg/ml and aphakia 255 pg/ml.
This is the first published report to show that lens protein is measurable in serum and to demonstrate the feasibility of using serum assays of lens proteins to gain information on the physiological status of the lens. Our results confirm the hypothesis that molecular and cellular events leading to cataract cause increased leakiness of lens cell membranes with release of lens proteins appearing in the blood. It is conceivable that measurement of lens proteins in serum might find future use in the evaluation of cataract risk, potentially cataractogenic and anticataractogenic agents, retained lens fragments after phacoemulsification, secondary cataract, phacolytic glaucoma, anaphylactic endophthalmitis, eye injuries, and other eye diseases.
确定检测血清中的晶状体蛋白是否是获取人类晶状体生理状态信息的可行方法。
采用夹心放射免疫分析法测定了280名年龄在25至94岁之间受试者血清中的γ-晶状体蛋白浓度。查阅医疗记录以诊断白内障和无晶状体眼。
年龄和性别对血清γ-晶状体蛋白浓度无影响。有57名白内障患者和27名无晶状体眼患者。所有白内障组的γ-晶状体蛋白水平较高，无晶状体眼组的γ-晶状体蛋白水平较低。选定受试者组的平均γ-晶状体蛋白浓度如下：透明晶状体301 pg/ml；纯核性白内障344 pg/ml；纯皮质性白内障439 pg/ml；无晶状体眼255 pg/ml。
这是首篇发表的报告，表明血清中可检测到晶状体蛋白，并证明了利用血清晶状体蛋白检测获取晶状体生理状态信息的可行性。我们的结果证实了这一假设，即导致白内障的分子和细胞事件会使晶状体细胞膜通透性增加，导致晶状体蛋白释放入血。可以想象，血清中晶状体蛋白的检测未来可能用于评估白内障风险、潜在的致白内障和抗白内障药物、超声乳化术后残留的晶状体碎片、后发性白内障、晶状体溶解性青光眼、过敏性眼内炎、眼外伤及其他眼部疾病。
