Reynolds J S, Troy T L, Mayer R H, Thompson A B, Waters D J, Cornell K K, Snyder P W, Sevick-Muraca E M
School of Chemical Engineering, Purdue University, West Lafayette, IN 47907, USA.
Photochem Photobiol. 1999 Jul;70(1):87-94.
We present near-infrared frequency-domain photon migration imaging for the lifetime sensitive detection and localization of exogenous fluorescent contrast agents within tissue-simulating phantoms and actual tissues. We employ intensity-modulated excitation light that is expanded and delivered to the surface of a tissue or tissue-simulating phantom. The intensity-modulated fluorescence generated from within the volume propagates to the surface and is collected using a gain-modulated image-intensified charge-coupled device camera. From the spatial values of modulation amplitude and phase of the detected fluorescent light, micromolar volumes of diethylthiatricarbocyanine iodide (tau = 1.17 ns) and indocyanine green (ICG) (tau = 0.58 ns) embedded 1.0 cm deep in a tissue phantom are localized and discriminated on the basis of their lifetime differences. To demonstrate the utility of frequency-domain fluorescent measurements for imaging disease, we image the fluorescence emitted from the surface of in vivo and ex vivo canine mammary gland tissues containing lesions with preferential uptake of ICG. Pathology confirms the ability to detect spontaneous mammary tumors and regional lymph nodes amidst normal mammary tissue and fat as deep as 1.5 cm from the tissue surface.
我们展示了用于在组织模拟体模和实际组织中对外源性荧光造影剂进行寿命敏感检测和定位的近红外频域光子迁移成像。我们采用强度调制的激发光，该光被扩展并传输到组织或组织模拟体模的表面。从体积内部产生的强度调制荧光传播到表面，并使用增益调制的图像增强电荷耦合器件相机进行采集。根据检测到的荧光的调制幅度和相位的空间值，可对嵌入组织体模中1.0厘米深处的微摩尔体积的二乙硫代三碳菁碘化物（τ = 1.17纳秒）和吲哚菁绿（ICG）（τ = 0.58纳秒）基于它们的寿命差异进行定位和区分。为了证明频域荧光测量在疾病成像中的效用，我们对体内和体外含有优先摄取ICG的病变的犬乳腺组织表面发出的荧光进行成像。病理学证实了在距组织表面深达1.5厘米的正常乳腺组织和脂肪中检测自发乳腺肿瘤和区域淋巴结的能力。
