经基因工程改造以分泌白细胞介素-15的肿瘤细胞可增强体内抗肿瘤免疫反应。
Tumour cells engineered to secrete interleukin-15 augment anti-tumour immune responses in vivo.
作者信息
Hazama S, Noma T, Wang F, Iizuka N, Ogura Y, Yoshimura K, Inoguchi E, Hakozaki M, Hirose K, Suzuki T, Oka M
机构信息
Department of Surgery II, Yamaguchi University School of Medicine, Ube, Japan.
出版信息
Br J Cancer. 1999 Jul;80(9):1420-6. doi: 10.1038/sj.bjc.6690538.
Abstract
We examined the effect of interleukin-15 (IL-15) gene transfer into tumour cells on the host's anti-tumour response. In BALB/c mice IL-15 producing Meth-A cells (Meth-A/IL-15) underwent complete rejection, in a response characterized by massive infiltration of CD4+ T-cells and neutrophils. In contrast, Meth-A cells transfected with vector alone (Meth-A/Neo) grew rapidly. Moreover, rechallenged parental cells also were rejected in association with CD8* T-cell infiltration. However, in nude mice there was no drastic difference between Meth-A/IL-15 and Meth-A/Neo cells. These results demonstrate that IL-15-secreting tumour cells can stimulate local and systemic T-cell-dependent immunity and therefore may have a potential role in cancer therapy.
摘要
我们研究了将白细胞介素-15（IL-15）基因导入肿瘤细胞对宿主抗肿瘤反应的影响。在BALB/c小鼠中，产生IL-15的Meth-A细胞（Meth-A/IL-15）完全被排斥，其反应特征是CD4+ T细胞和中性粒细胞大量浸润。相比之下，仅用载体转染的Meth-A细胞（Meth-A/Neo）生长迅速。此外，再次接种的亲本细胞也与CD8* T细胞浸润一起被排斥。然而，在裸鼠中，Meth-A/IL-15和Meth-A/Neo细胞之间没有显著差异。这些结果表明，分泌IL-15的肿瘤细胞可以刺激局部和全身的T细胞依赖性免疫，因此可能在癌症治疗中具有潜在作用。
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