Sen B H, Safavi K E, Spångberg L S
Department of Restorative Dentistry and Endodontology, School of Dentistry, Ege University, Turkey.
J Endod. 1999 Apr;25(4):235-8. doi: 10.1016/S0099-2399(99)80149-6.
The purpose of this study was to evaluate the antifungal properties of 0.12% chlorhexidine, 1% NaOCl, and 5% NaOCl. Root sections were enlarged and the smear layer was removed in half of the specimens. The specimens were fixed in the wells of tissue culture plates. Each root canal was dispensed with an inoculum of Candida albicans. After 10 days, the root sections were treated with 3 ml of either disinfectant solution for 1 min, 5 min, 30 min, and 1 h. Then, root sections were incubated in test tubes having Sabouraud's Dextrose Broth at 37 degrees C for 24 h. In the presence of the smear layer, antifungal activity was observed only in 1-h treatment groups for all solutions. However, in the absence of the smear layer, 5% NaOCl alone started to show antifungal activity after 30 min. The antimicrobial effectiveness of irrigating solutions should be re-evaluated, particularly in patients predisposed to oral candidiasis.
本研究的目的是评估0.12%氯己定、1%次氯酸钠和5%次氯酸钠的抗真菌特性。在一半的标本中扩大牙根断面并去除玷污层。将标本固定在组织培养板的孔中。每个根管接种白色念珠菌。10天后，用3毫升任一种消毒溶液对牙根断面处理1分钟、5分钟、30分钟和1小时。然后，将牙根断面在装有沙氏葡萄糖肉汤的试管中于37℃孵育24小时。在有玷污层的情况下，所有溶液仅在1小时处理组观察到抗真菌活性。然而，在无玷污层的情况下，仅5%次氯酸钠在30分钟后开始显示抗真菌活性。冲洗液的抗菌效果应重新评估，尤其是在易患口腔念珠菌病的患者中。
