β-半乳糖苷酶在黑腹果蝇细胞培养物中由激素诱导产生。
beta-Galactosidase is induced by hormone in Drosophila melanogaster cell cultures.
作者信息
Best-Belpomme M, Courgeon A M, Rambach A
出版信息
Proc Natl Acad Sci U S A. 1978 Dec;75(12):6102-6. doi: 10.1073/pnas.75.12.6102.
Abstract
Drosophila melanogaster cell lines Kc and Ca and clones FC and RF6, cultured in vitro, have no detectable beta-galactosidase (beta-galactoside galactohydrolase, EC 3.2.1.23) activity (as measured by hydrolysis of o-nitrophenyl-beta-D-galoctoside). Ecdysterone, a hormonal steroid of critical importance in insect physiology, clearly induces beta-galactosidase activity in D. melanogaster cells cultured in vitro. Induction occurs in cell lines or clones known to be sensitive to ecdysterone (K, Ca, and Fc) and does not occur in clones known to be resistant to the hormone (RF6). Some properties of the hormone-induced beta-galactosidase activity were studied. The Km for o-nitrophenyl galactoside is 0.35 mM and the Ki for lactose is 12 mM (similar to those of Escherichia coli beta-galactosidase); the activity can be recovered after sodium dodecyl sulfate treatment; the enzyme is a tetramer (Mr of the monomer is 64,000).
摘要
在体外培养的果蝇细胞系Kc和Ca以及克隆FC和RF6，没有可检测到的β-半乳糖苷酶（β-半乳糖苷半乳糖水解酶，EC 3.2.1.23）活性（通过邻硝基苯基-β-D-半乳糖苷的水解来测量）。蜕皮甾酮是昆虫生理学中至关重要的一种激素类固醇，它能明显诱导体外培养的果蝇细胞中的β-半乳糖苷酶活性。诱导作用发生在已知对蜕皮甾酮敏感的细胞系或克隆（K、Ca和Fc）中，而在已知对该激素有抗性的克隆（RF6）中则不发生。对激素诱导的β-半乳糖苷酶活性的一些特性进行了研究。邻硝基苯基半乳糖苷的Km为0.35 mM，乳糖的Ki为12 mM（与大肠杆菌β-半乳糖苷酶的相似）；该活性在十二烷基硫酸钠处理后仍可恢复；该酶是一种四聚体（单体的Mr为64,000）。
相似文献
1
beta-Galactosidase is induced by hormone in Drosophila melanogaster cell cultures.β-半乳糖苷酶在黑腹果蝇细胞培养物中由激素诱导产生。
Proc Natl Acad Sci U S A. 1978 Dec;75(12):6102-6. doi: 10.1073/pnas.75.12.6102.
2
A critical period of ecdysterone action on sensitive clones of Drosophila cultured in vitro: the maturation of the cells.蜕皮甾酮对体外培养的果蝇敏感克隆作用的关键时期：细胞的成熟
Eur J Biochem. 1980 Nov;112(1):185-91. doi: 10.1111/j.1432-1033.1980.tb05000.x.
3
Catalase is induced by ecdysterone and ethanol in Drosophila cells.过氧化氢酶在果蝇细胞中由蜕皮激素和乙醇诱导产生。
Eur J Biochem. 1982 Jan;121(2):349-55. doi: 10.1111/j.1432-1033.1982.tb05793.x.
4
Ecdysterone selectively stimulates the expression of a 23000-Da heat-shock protein-beta-galactosidase hybrid gene in cultured Drosophila cells.蜕皮甾酮可选择性地刺激培养的果蝇细胞中一种23000道尔顿的热休克蛋白-β-半乳糖苷酶杂交基因的表达。
Dev Biol. 1985 Aug;110(2):321-30. doi: 10.1016/0012-1606(85)90091-0.
5
Purification and properties of an inducible beta-galactosidase isolated from the yeast Kluyveromyces lactis.从乳酸克鲁维酵母中分离出的一种可诱导β-半乳糖苷酶的纯化及性质
J Bacteriol. 1979 Jan;137(1):51-61. doi: 10.1128/jb.137.1.51-61.1979.
6
[Comparison of the effects of alpha and beta ecdysone on a diploid clone of cultured Drosophila melanogaster cells in vitro: induction of proteins and morphological changes].[α-蜕皮激素和β-蜕皮激素对体外培养的黑腹果蝇细胞二倍体克隆的影响比较：蛋白质诱导及形态学变化]
C R Acad Hebd Seances Acad Sci D. 1976 Jul 12;283(2):155-8.
7
Heat shock and ecdysterone activation of the Drosophila melanogaster hsp23 gene; a sequence element implied in developmental regulation.果蝇hsp23基因的热休克和蜕皮激素激活；一个与发育调控相关的序列元件
EMBO J. 1986 Jul;5(7):1667-73. doi: 10.1002/j.1460-2075.1986.tb04410.x.
8
Aggregation properties of beta-galactosidase of human urine and degradation of its natural substrates by a purified preparation of the enzyme.人尿β-半乳糖苷酶的聚集特性及其纯化制剂对天然底物的降解作用。
Biochim Biophys Acta. 1982 May 21;704(1):134-43. doi: 10.1016/0167-4838(82)90140-6.
9
Physiological studies of beta-galactosidase induction in Kluyveromyces lactis.乳酸克鲁维酵母中β-半乳糖苷酶诱导的生理学研究。
J Bacteriol. 1980 Jun;142(3):777-85. doi: 10.1128/jb.142.3.777-785.1980.
10
Induction of beta-galactosidase in Streptomyces violaceus.紫色链霉菌中β-半乳糖苷酶的诱导
Can J Microbiol. 1979 Jul;25(7):833-40. doi: 10.1139/m79-123.
引用本文的文献
1
Characterization of the activity of β-galactosidase from and in fixed and non-fixed tissues.来自[具体来源未给出]的β-半乳糖苷酶在固定和未固定的[具体组织未给出]组织中的活性表征。
Biochim Open. 2016 Jun 22;3:1-7. doi: 10.1016/j.biopen.2016.06.001. eCollection 2016 Dec.
2
The morphological response of Kc-H cells to ecdysteroids: Hormonal specificity.Kc-H细胞对蜕皮甾体的形态学反应：激素特异性。
Wilehm Roux Arch Dev Biol. 1980 Feb;189(1):1-15. doi: 10.1007/BF00848562.
3
The effect of cytoskeletal disrupting agents on the morphological response of a clonedManduca sexta cell line to 20-hydroxy-ecdysone.细胞骨架破坏剂对克隆的烟草天蛾细胞系对20-羟基蜕皮激素形态学反应的影响。
Wilehm Roux Arch Dev Biol. 1981 May;190(3):150-155. doi: 10.1007/BF00867801.
4
cDNA clones for the ecdysone-inducible polypeptide (EIP) mRNAs of Drosophila Kc cells.果蝇 Kc 细胞蜕皮激素诱导多肽（EIP）mRNA 的 cDNA 克隆。
EMBO J. 1984 Jan;3(1):235-43. doi: 10.1002/j.1460-2075.1984.tb01790.x.
5
Acetylcholinesterase activity in an Aedes aegypti cell line.埃及伊蚊细胞系中的乙酰胆碱酯酶活性。
Experientia. 1981 Apr 15;37(4):429-31. doi: 10.1007/BF01959903.
6
Effect of an ecdysteroid on DNA synthesis, DNA polymerase a and thymidine kinase activities of Drosophila melanogaster cells in vitro.一种蜕皮甾体对体外培养的黑腹果蝇细胞DNA合成、DNA聚合酶α和胸苷激酶活性的影响。
Experientia. 1982 Oct 15;38(10):1197-9. doi: 10.1007/BF01959738.
7
Drosophila cells and ecdysterone: a model system for gene regulation.果蝇细胞与蜕皮激素：基因调控的模型系统
In Vitro. 1984 Dec;20(12):959-74. doi: 10.1007/BF02619669.
8
Cytogenic mapping and isolation of mutations of the beta-Gal-1 locus of Drosophila melanogaster.黑腹果蝇β - Gal - 1基因座的细胞遗传学定位与突变分离
Mol Gen Genet. 1984;198(2):75-83. doi: 10.1007/BF00328704.
9
Chemical selection for beta-galactosidase activity in Drosophila melanogaster.黑腹果蝇中β-半乳糖苷酶活性的化学筛选。
Biochem Genet. 1987 Jun;25(5-6):327-33. doi: 10.1007/BF00554542.
10
1731, a new retrotransposon with hormone modulated expression.1731，一种具有激素调节表达的新型逆转座子。
Nucleic Acids Res. 1986 Nov 25;14(22):9017-33. doi: 10.1093/nar/14.22.9017.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
A method for determining the sedimentation behavior of enzymes: application to protein mixtures.一种测定酶沉降行为的方法：应用于蛋白质混合物
J Biol Chem. 1961 May;236:1372-9.
3
Genetic regulatory mechanisms in the synthesis of proteins.蛋白质合成中的遗传调控机制。
J Mol Biol. 1961 Jun;3:318-56. doi: 10.1016/s0022-2836(61)80072-7.
4
Biochemistry of the sphingolipides. VII. Structure of the cerebrosides.鞘脂类的生物化学。VII. 脑苷脂的结构。
J Biol Chem. 1952 Nov;199(1):283-8.
5
Polypeptides of the tail fibres of bacteriophage T4.噬菌体T4尾丝的多肽
J Mol Biol. 1971 Dec 28;62(3):465-77. doi: 10.1016/0022-2836(71)90148-3.
6
In vitro culture of Drosophila melanogaster embryonic cells.黑腹果蝇胚胎细胞的体外培养。
In Vitro. 1970 Nov-Dec;6(3):162-72. doi: 10.1007/BF02617759.
7
[Isolation, in tissue culture, of Drosophila melangaster cell lines].[在组织培养中分离黑腹果蝇细胞系]
C R Acad Hebd Seances Acad Sci D. 1969 Mar 31;268(13):1771-3.
8
Action of insect hormones at the cellular level. Morphological changes of a diploid cell line of Drosophila melanogaster, treated with ecdysone and several analogues in vitro.昆虫激素在细胞水平上的作用。体外经蜕皮激素及几种类似物处理的黑腹果蝇二倍体细胞系的形态变化。
Exp Cell Res. 1972 Oct;74(2):327-36. doi: 10.1016/0014-4827(72)90384-9.
9
Effects of - and -ecdysone on in vitro diploid cell multiplication in Drosophila melanogaster.α-蜕皮激素和β-蜕皮激素对黑腹果蝇体外二倍体细胞增殖的影响。
Nat New Biol. 1972 Aug 23;238(86):250-1. doi: 10.1038/newbio238250a0.
10
Cerebroside sulphatase activity of arylsulphatases from various invertebrates.各种无脊椎动物芳基硫酸酯酶的脑苷脂硫酸酯酶活性。
Hoppe Seylers Z Physiol Chem. 1974 Jan;355(1):33-44. doi: 10.1515/bchm2.1974.355.1.33.
Zotero 插件