Hawken R J, Murtaugh J, Flickinger G H, Yerle M, Robic A, Milan D, Gellin J, Beattie C W, Schook L B, Alexander L J
Program on Comparative Genomics, College of Veterinary Medicine, University of Minnesota, 1365 Gortner Avenue, St. Paul, Minnesota 55108, USA.
Mamm Genome. 1999 Aug;10(8):824-30. doi: 10.1007/s003359901097.
A whole-genome radiation hybrid (WG-RH) panel was used to generate a first-generation radiation map of the porcine (Sus scrofa) genome. Over 900 Type I and II markers were used to amplify the INRA-University of Minnesota porcine Radiation Hybrid panel (IMpRH) comprised of 118 hybrid clones. Average marker retention frequency of 29.3% was calculated with 757 scorable markers. The RHMAP program established 128 linkage groups covering each chromosome (n = 19) at a lod >/= 4.8. Fewer than 10% of the markers (59) could not be placed within any linkage group at a lod score >/=4.8. Linkage group order for each chromosome was determined by incorporating linkage data from the swine genetic map as well as physical assignments. The current map has an estimated ratio of approximately 70 kb/cR and a maximum theoretical resolution of 145 kb. This initial map forms a template for establishing accurate YAC and BAC contigs and eventual positional cloning of genes associated with complex traits.
利用全基因组辐射杂种（WG-RH）图谱构建了猪（Sus scrofa）基因组的第一代辐射图谱。使用900多个I型和II型标记对由118个杂种克隆组成的法国国家农业研究院-明尼苏达大学猪辐射杂种图谱（IMpRH）进行扩增。用757个可评分标记计算出平均标记保留频率为29.3%。RHMAP程序在lod≥4.8时建立了覆盖每条染色体（n = 19）的128个连锁群。在lod得分≥4.8时，不到10%的标记（59个）无法置于任何连锁群中。通过整合猪遗传图谱的连锁数据以及物理定位，确定了每条染色体的连锁群顺序。当前图谱的估计比率约为70 kb/cR，最大理论分辨率为145 kb。这一初始图谱为建立准确的酵母人工染色体（YAC）和细菌人工染色体（BAC）重叠群以及最终对与复杂性状相关的基因进行定位克隆提供了模板。
