Butaye P, Devriese L A, Haesebrouck F
Faculty of Veterinary Medicine, Department of Pathology, Bacteriology and Poultry Disease, University of Ghent, Merelbeke, Belgium.
Microb Drug Resist. 1999 Summer;5(2):131-4. doi: 10.1089/mdr.1999.5.131.
Isolation results of vancomycin-resistant enterococci (VRE) of fecal samples from pigs and broiler and layer chickens obtained with two vancomycin-supplemented enrichment media, kanamycin aesculin azide (KAA) broth and Enterococcosel (ECC) broth, and three isolation media, KAA agar, ECC agar, and Slanetz and Bartley (SL) agar, were compared. Direct isolation on vancomycin-containing agar plates was not efficient in swine and layer chickens, which had only low numbers of VRE. In broilers chickens, the VRE content of the samples was high, and SL as well as ECC were found to perform better than KAA agar. The same three agar media were used as selective plating media after 1 and 2 days incubation of the samples in KAA and ECC enrichment broths. Sensitivities of the 12 different enrichment-plate combinations tested ranged from 0 to 81% in layer chickens and from 5 to 44% in samples from pigs. In the high prevalence type of samples from broilers, sensitivities still varied substantially from 52 to 78%. Incubating vancomycin-containing enrichment broths for 2 days compared with 1 day was favorable for the isolation of vancomycin-resistant Enterococcus faecalis, E. gallinarum, and E. casseliflavus but not for E. faecium and E. hirae/E. durans. ECC broth and ECC plates yielded the highest number of E. gallinarum and E. casseliflavus. In layer as well as in broiler chickens, ECC broth incubated for 2 days and plated on ECC agar was the most sensitive method. In pigs, however, KAA broth incubated for 2 days and plated on ECC medium yielded the highest number of VRE.
比较了用两种添加万古霉素的增菌培养基（卡那霉素七叶苷叠氮化物（KAA）肉汤和肠球菌琼脂（ECC）肉汤）以及三种分离培养基（KAA琼脂、ECC琼脂和斯兰茨-巴特利（SL）琼脂）从猪、肉鸡和蛋鸡粪便样本中分离耐万古霉素肠球菌（VRE）的结果。在猪和蛋鸡中，直接在含万古霉素的琼脂平板上分离效率不高，它们的VRE数量很少。在肉鸡中，样本的VRE含量很高，发现SL和ECC的表现优于KAA琼脂。在KAA和ECC增菌肉汤中孵育样本1天和2天后，使用相同的三种琼脂培养基作为选择性平板培养基。在蛋鸡中，测试的12种不同增菌-平板组合的敏感性范围为0%至81%，在猪的样本中为5%至44%。在肉鸡的高流行类型样本中，敏感性仍有很大差异，为52%至78%。与孵育1天相比，将含万古霉素的增菌肉汤孵育2天有利于分离耐万古霉素的粪肠球菌、鹑鸡肠球菌和格氏肠球菌，但不利于分离屎肠球菌和平肠球菌/耐久肠球菌。ECC肉汤和ECC平板分离出的鹑鸡肠球菌和格氏肠球菌数量最多。在蛋鸡和肉鸡中，将ECC肉汤孵育2天并接种在ECC琼脂上是最敏感的方法。然而，在猪中，将KAA肉汤孵育2天并接种在ECC培养基上产生的VRE数量最多。
