Chu N S, Chan-Myers H, Ghazanfari N, Antonoplos P
Advanced Sterilization Products, Irvine, CA 92618-9824, USA.
Am J Infect Control. 1999 Aug;27(4):315-9. doi: 10.1016/s0196-6553(99)70050-7.
Surgical instruments exposed to sterile body sites should be contaminated with relatively low levels of microbial contamination or bioburden; however, few studies in the literature have determined the quantitative level and types of contamination. A study was conducted at 2 clinical sites to determine the level of microbial contamination of surgical instruments after clinical use and after washing. Quantitative assays showed that bioburden levels were in the range of 0 to 4415 colony forming units per instrument after clinical use, and 88% of the instruments had bioburden levels lower than 1000. As expected, a reduction in counts occurred after washing; however, in some cases, higher counts were found on the instruments after the washing process. Although the washing procedure is effective in reducing the microbial levels deposited on the surgical instruments during use, a recontamination process occurs that results in increased counts after washing. The low bioburden level after washing consists of predominantly vegetative microorganisms that present a relatively low challenge to sterilization and disinfection systems.
暴露于无菌身体部位的手术器械应受到相对较低水平的微生物污染或生物负载；然而，文献中很少有研究确定污染的定量水平和类型。在2个临床地点进行了一项研究，以确定临床使用后和清洗后手术器械的微生物污染水平。定量分析表明，临床使用后每台器械的生物负载水平在0至4415菌落形成单位范围内，88%的器械生物负载水平低于1000。正如预期的那样，清洗后计数减少；然而，在某些情况下，清洗过程后器械上的计数更高。虽然清洗程序有效地降低了使用过程中沉积在手术器械上的微生物水平，但会发生再污染过程，导致清洗后计数增加。清洗后较低的生物负载水平主要由营养微生物组成，这些微生物对灭菌和消毒系统构成的挑战相对较低。
