Chan-Myers H, McAlister D, Antonoplos P
Advanced Sterilization Products, Irvine, CA 92618-9824, USA.
Am J Infect Control. 1997 Dec;25(6):471-6. doi: 10.1016/s0196-6553(97)90070-5.
Controversy exists concerning the degree of microbial contamination associated with the use of rigid lumened medical devices, the efficacy of standard cleaning techniques used to remove pathogenic microorganisms from lumen channels, and whether patients are placed at risk of cross infection because of microbial contamination. In this study the level and types of microorganisms found on rigid lumened medical devices before and after cleaning in a hospital environment were investigated. The bioburden level after clinical use was found to be relatively low, ranging from 10(1) to 10(4) colony forming units (CFU) per device. After the instruments were cleaned, none of the devices studied contained bioburden levels greater than 10(4) CFU and 83% had bioburden levels less than or equal to 10(2) CFU. The bioburden present before cleaning was comprised of organisms derived from the handling of the device, from the hospital environment, and from the patient. The bioburden present after cleaning was comprised of organisms typically derived from the handling of the device and from the hospital environment. The level of bioburden per device was also related to the anatomic site where the device was used, with lower numbers of organisms found on devices exposed to sterile body sites and the respiratory tract.
关于使用带内腔的硬质医疗器械所涉及的微生物污染程度、用于从内腔通道清除致病微生物的标准清洁技术的有效性以及患者是否因微生物污染而面临交叉感染风险，目前存在争议。在本研究中，对医院环境中带内腔的硬质医疗器械清洁前后所发现的微生物水平和类型进行了调查。临床使用后的生物负载水平相对较低，每个器械的菌落形成单位（CFU）为10¹至10⁴个。器械清洁后，所研究的器械中没有一个的生物负载水平超过10⁴CFU，83%的器械生物负载水平小于或等于10²CFU。清洁前存在的生物负载由来自器械操作、医院环境和患者的微生物组成。清洁后存在的生物负载由通常来自器械操作和医院环境的微生物组成。每个器械的生物负载水平还与器械使用的解剖部位有关，暴露于无菌身体部位和呼吸道的器械上发现的微生物数量较少。
