Escola de Enfermagem, Universidade de São Paulo , São Paulo, SP , Brasil.
Braz J Microbiol. 2009 Jul;40(3):541-6. doi: 10.1590/S1517-838220090003000016. Epub 2009 Sep 1.
It is estimated that electric drills (ED) have been used in orthopaedic surgeries for bone drilling for more than 50 years in Brazilian hospitals. It is an electric, thermosensitive equipment, not indicated for surgical use, which has not been previously evaluated regarding the sterilization efficacy, being suspect of infection risk. This study evaluated the efficacy of sterilization by ethylene oxide (EtO) of new drills that were intentionally contaminated with Bacillus atrophaeus spores. An experimental, laboratory, randomized applied research was developed, where 16 electric drills were analyzed, in addition to positive and negative controls. All the previously cleaned and sterilized equipment were submitted to contamination by spores. The experimental group was submitted to cleaning and sterilization by EtO and test of sterility by filtration through a 0.45 >m membrane. The membranes were cultivated and Gram and Wirtz-Conklin staining were carried out in positive results for spore visualization. An efficacy of 99.99999881% of the process of sterilization by EtO was confirmed, with a probability of survival of 1.19 x 10 (-8). Under the development conditions of the experiment, the efficacy of the sterilization of ED by EtO was confirmed.
据估计，电动钻头（ED）在巴西医院的骨科手术中已经用于骨钻超过 50 年。它是一种电动、热敏设备，不用于手术，之前没有评估过其消毒效果，存在感染风险。本研究评估了环氧乙烷（EtO）对新钻头的消毒效果，这些钻头故意被萎缩芽孢杆菌孢子污染。这是一项实验性、实验室、随机应用研究，其中分析了 16 个电钻，以及阳性和阴性对照。所有之前清洁和消毒的设备都被孢子污染。实验组接受 EtO 的清洁和消毒，并通过 0.45μm 膜过滤进行无菌性测试。将膜进行培养，并对阳性结果进行革兰氏染色和 Wirtz-Conklin 染色，以观察孢子的可视化。EtO 消毒过程的有效性为 99.99999881%，存活概率为 1.19 x 10(-8)。在实验的发展条件下，确认了 EtO 对 ED 消毒的有效性。
