Donaldson M T, Palmer J E
Department of Clinical Studies, New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, Kennett Square 19348, USA.
J Am Vet Med Assoc. 1999 Aug 1;215(3):358-61.
To determine prevalence of clostridial enterotoxins in feces of horses with diarrhea and colic, and to determine whether an association exists between detection of clostridial enterotoxins in feces and development of diarrhea as a complication of colic.
Prospective case series and case-control study.
174 horses with diarrhea, colic, or problems not related to the gastrointestinal tract.
Horses were assigned to 1 of 4 groups: colic with diarrhea (group 1; n = 30); colic without diarrhea (group 2; 30); diarrhea without colic (group 3; 57); and control (group 4; 57). Feces were evaluated by use of ELISA to detect Clostridium perfringens enterotoxin (CPE) and C difficile toxin A (TOXA). Frequency of detection of CPE or TOXA in groups 1 and 3 was compared with that in groups 2 and 4, respectively.
Prevalence of enteric clostridiosis in horses in group 3 was 25%. Clostridium perfringens enterotoxin was detected in 9 of 57 (16%), TOXA in 8 of 57 (14%), and both toxins in 3 of 57 (5%) fecal samples collected from these horses. Neither toxin was detected in feces of the age-matched horses in group 4. Clostridial enterotoxins were detected in feces of 7 of 60 (12%) horses with colic (groups 1 and 2), however, a significant association was not found between detection of enterotoxins in feces and development of diarrhea as a complication of colic.
Clostridia are important etiologic agents of diarrhea in horses. Additionally, changes in intestinal flora of horses with colic may allow for proliferation of clostridia and elaboration of enterotoxins regardless of whether diarrhea develops.
确定腹泻和绞痛马匹粪便中梭菌肠毒素的流行情况，并确定粪便中梭菌肠毒素的检测与作为绞痛并发症的腹泻发生之间是否存在关联。
前瞻性病例系列和病例对照研究。
174匹患有腹泻、绞痛或与胃肠道无关问题的马。
将马分为4组中的1组：伴有腹泻的绞痛（第1组；n = 30）；无腹泻的绞痛（第2组；30匹）；无绞痛的腹泻（第3组；57匹）；以及对照组（第4组；57匹）。使用酶联免疫吸附测定法（ELISA）评估粪便，以检测产气荚膜梭菌肠毒素（CPE）和艰难梭菌毒素A（TOXA）。分别将第1组和第3组中CPE或TOXA的检测频率与第2组和第4组中的进行比较。
第3组马匹中肠道梭菌病的流行率为25%。从这些马匹收集的57份粪便样本中，57份中有9份（16%）检测到产气荚膜梭菌肠毒素，57份中有8份（14%）检测到TOXA，57份中有3份（5%）同时检测到两种毒素。第4组年龄匹配的马匹粪便中均未检测到毒素。60匹患有绞痛的马匹（第1组和第2组）中有7匹（12%）粪便中检测到梭菌肠毒素，然而，粪便中肠毒素的检测与作为绞痛并发症的腹泻发生之间未发现显著关联。
梭菌是马匹腹泻的重要病因。此外，患有绞痛的马匹肠道菌群的变化可能会使梭菌增殖并产生肠毒素，无论是否发生腹泻。
