采用高效液相色谱-高效薄层色谱法对肝脏中西马特罗和克伦特罗残留量进行分析。

Cimaterol and clenbuterol residue analysis by HPLC-HPTLC in liver.

作者信息

Degroodt J M, Wyhowski de Bukanski B, De Groof J, Beernaert H

机构信息

Ministère de la Santé Publique et de l'Environnement, Brussels, Belgium.

出版信息

Z Lebensm Unters Forsch. 1991 May;192(5):430-2. doi: 10.1007/BF01193142.

DOI:10.1007/BF01193142

PMID:1676204

Abstract

A method has been developed for the analysis of cimaterol and clenbuterol residues in liver, with detection limits of 0.25 micrograms/kg and 0.5 micrograms/kg, respectively. The recovery varied from 55% to 60%. After extraction, a clean-up procedure with Baker-spe C-18 columns was performed. The two chemical compounds of interest were eluted with methanol. Cimaterol and clenbuterol were quantitatively determined by high-performance liquid chromatography (HPLC) using an RP-Select B (5 microns) column and a post-column reaction procedure. The positive results were confirmed by high-performance thin-layer chromatography (HPTLC) as this technique reaches the same level of sensitivity as the HPLC method.

摘要

已开发出一种分析肝脏中克伦特罗和西马特罗残留量的方法，其检测限分别为0.25微克/千克和0.5微克/千克。回收率在55%至60%之间。提取后，采用Baker-spe C-18柱进行净化处理。用甲醇洗脱两种目标化合物。使用RP-Select B（5微米）柱和柱后反应程序，通过高效液相色谱法（HPLC）对克伦特罗和西马特罗进行定量测定。阳性结果通过高效薄层色谱法（HPTLC）确认，因为该技术达到了与HPLC方法相同的灵敏度水平。