Wu W, Kuang P, Li Z
Department of Neurology, Chinese PLA General Hospital, Postgraduate Military Medical School, Beijing.
J Tradit Chin Med. 1997 Sep;17(3):220-5.
We have found that Radix Salviae Miltiorrhizae (RSM) plays a protective role in ischemic brain injury, which attracted us to investigate the effect of RSM on apoptosis of neurons during cerebral ischemia and reperfusion. The apoptotic cells in ischemic brains at different reperfusion intervals were tested with the method of TdT-mediated dUTP-DIG nick end labeling (TUNEL), and the effect of RSM on the apoptosis of neurons was studied in left middle cerebral artery (LMCA) occlusion in rat models (n = 18). The results showed that few scattered apoptotic cells were observed in right cerebral hemisphere after LMCA occlusion and reperfusion, and that a lot of apoptotic cells were found in left ischemic cerebral cortex and caudoputamen at 12 h reperfusion, and they reached peak at 24-48 h reperfusion. However, in rats pretreated with RSM, the number of apoptotic cells in left cortex and caudoputamen reduced significantly and the neuronal damage was much milder at 24 h reperfusion as compared with those of saline-treated rats. From this study, we conclude that administration of RSM can reduce the apoptotic of neurons induced by cerebral ischemia and reperfusion and afford significant cerebroprotection in the model of focal cerebral ischemia and reperfusion.
我们发现丹参对缺血性脑损伤具有保护作用，这促使我们研究丹参在脑缺血再灌注过程中对神经元凋亡的影响。采用TdT介导的dUTP-DIG缺口末端标记法（TUNEL）检测不同再灌注时间间隔下缺血脑内的凋亡细胞，并在大鼠大脑中动脉闭塞模型（n = 18）中研究丹参对神经元凋亡的影响。结果显示，大脑中动脉闭塞再灌注后，右侧大脑半球可见少量散在的凋亡细胞，再灌注12小时时，左侧缺血大脑皮质和尾壳核出现大量凋亡细胞，并在再灌注24 - 48小时达到高峰。然而，与生理盐水处理的大鼠相比，丹参预处理的大鼠在再灌注24小时时，左侧皮质和尾壳核的凋亡细胞数量显著减少，神经元损伤也明显减轻。从本研究中，我们得出结论，给予丹参可减少脑缺血再灌注诱导的神经元凋亡，并在局灶性脑缺血再灌注模型中提供显著的脑保护作用。
