Nargi F, Kramer E, Mezencio J, Zamparo J, Whetstone C, Van Regenmortel M H, Briand J P, Muller S, Brown F
Agricultural Research Service, USDA, Plum Island Animal Disease Center, Greenport, NY 11944, USA.
Vaccine. 1999 Jul 16;17(22):2888-93. doi: 10.1016/s0264-410x(99)00127-9.
Nine pigs were given a single inoculum of 100 microg of the all-D retro peptide corresponding to the immunodominant GH loop encompassing residues 141-159 of capsid protein VP1 of foot-and-mouth disease virus serotype A, sub-type 12. The peptide was conjugated to activated keyhole limpet haemocyanin and oil-adjuvanted before inoculation. The animals were challenged eleven weeks post-vaccination by exposing them to a pig which had been infected with the virus by inoculation. Two naive animals were included in the challenge study as controls. One of the vaccinated animals was completely unprotected and two developed very small lesions. None of the six remaining animals exhibited any clinical signs but two developed antibodies against nonstructural proteins indicating that replication of the virus had occurred. No evidence of replication could be detected in the remaining four animals, either by rise in neutralizing antibody titre or by production of antibodies against non-structural proteins specific for virus replication.
给9头猪单次接种100微克全D型逆转录肽，该肽对应于口蹄疫病毒A血清型12亚型衣壳蛋白VP1免疫显性GH环中包含第141 - 159位残基的区域。该肽在接种前与活化的钥孔血蓝蛋白偶联并添加油佐剂。在接种疫苗11周后，通过将这些动物暴露于一头经接种感染了该病毒的猪来进行攻毒试验。攻毒研究中纳入了2头未接种的动物作为对照。其中1头接种疫苗的动物完全未得到保护，另外2头出现了非常小的损伤。其余6头动物均未表现出任何临床症状，但有2头产生了针对非结构蛋白的抗体，表明病毒发生了复制。通过中和抗体滴度升高或针对病毒复制特异性非结构蛋白的抗体产生，在其余4头动物中均未检测到病毒复制的证据。
