Cannon J S, Nicholas J, Orenstein J M, Mann R B, Murray P G, Browning P J, DiGiuseppe J A, Cesarman E, Hayward G S, Ambinder R F
Department of Pharmacology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
J Infect Dis. 1999 Sep;180(3):824-8. doi: 10.1086/314956.
In order to characterize the expression of the viral interleukin-6 (vIL-6) homologue in various human herpesvirus 8 (HHV-8)-associated diseases, in situ hybridization and immunohistochemistry were applied to formalin-fixed specimens. These assays showed consistent expression of vIL-6 in primary effusion lymphomas and in a case of human immunodeficiency virus (HIV)-associated lymphadenopathy with a Castleman's disease-like appearance. In contrast, Kaposi's sarcoma specimens showed marked differences among specimens. In a consecutive series of specimens from the Johns Hopkins archives, vIL-6 expression was demonstrated in one of 13 cases. However, among 7 specimens selected from the AIDS Malignancy Bank because of their high levels of the T1.1 lytic transcript and virion production, vIL-6 expression was consistently demonstrated in infiltrating mononuclear cells and occasional spindle-shaped cells. Thus vIL-6 expression in clinical specimens correlates with other measures of the lytic viral cycle. Both assays generally give congruent results and are consistent with the possibility that vIL-6 expression plays a role in the pathogenesis of a variety of HHV-8-associated diseases.
为了描述病毒白细胞介素-6（vIL-6）同源物在各种人类疱疹病毒8型（HHV-8）相关疾病中的表达情况，对福尔马林固定的标本进行了原位杂交和免疫组织化学检测。这些检测显示，vIL-6在原发性渗出性淋巴瘤以及1例具有卡斯特曼病样外观的人类免疫缺陷病毒（HIV）相关淋巴结病中持续表达。相比之下，卡波西肉瘤标本在不同标本之间表现出明显差异。在约翰霍普金斯医院存档的一系列连续标本中，13例中有1例显示vIL-6表达。然而，在从艾滋病恶性肿瘤库中选取的7个标本中，由于其T1.1裂解转录本水平高和病毒体产生量高，vIL-6在浸润的单核细胞和偶尔的梭形细胞中持续表达。因此，临床标本中vIL-6的表达与裂解病毒周期的其他指标相关。这两种检测通常给出一致的结果，并且与vIL-6表达在多种HHV-8相关疾病的发病机制中起作用的可能性一致。
