Kundu S N, Mitra K, Khuda Bukhsh A R
Department of Zoology, Kalyani University, Kalyani, India.
Complement Ther Med. 2000 Sep;8(3):157-65. doi: 10.1054/ctim.2000.0390.
To examine if the potentized homeopathic drug Arsenicum Album-30 can help restore the damage produced in protein profiles, DNA and RNA contents in liver and testis as a result of arsenic treatment in mice.
Sets of mice were injected with arsenic trioxide, one set was fed with Ars. Alb-30, another with Alcohol-30 and the final set was fed neither. The gel electrophoretic protein profiles and DNA and RNA contents in these three sets were studied.
Protein profiles were studied by SDS-PAGE method; the DNA and RNA contents were assayed by the standard methods through diphenylamine and orcinol reactions respectively.
arsenic trioxide injection produced some pathological conditions, drastic changes (mainly reduction of protein bands) in protein sub-fractions, reduced DNA and RNA contents in both liver and testis; Ars. Alb-30-fed arsenic-intoxicated mice showed revival and restoration in both liver and testis as revealed by gel patterns and quantitative assay of DNA and RNA.
Efficacy of the homeopathic drug Ars. Alb-30 in reducing arsenic-induced damage to protein and nucleic acids is substantiated and the mechanism of action of the homeopathic drug through expression of regulatory genes inferred.
研究高稀释顺势疗法药物白砷-30是否有助于恢复因砷处理小鼠而导致的肝脏和睾丸中蛋白质谱、DNA和RNA含量的损伤。
将几组小鼠注射三氧化二砷，一组喂食白砷-30，另一组喂食酒精-30，最后一组不喂食任何物质。研究这三组小鼠的凝胶电泳蛋白质谱以及DNA和RNA含量。
通过SDS-PAGE方法研究蛋白质谱；分别通过二苯胺和苔黑酚反应，采用标准方法测定DNA和RNA含量。
注射三氧化二砷导致了一些病理状况，蛋白质亚组分出现剧烈变化（主要是蛋白条带减少），肝脏和睾丸中的DNA和RNA含量降低；喂食白砷-30的砷中毒小鼠，通过凝胶图谱以及DNA和RNA的定量分析表明，其肝脏和睾丸均出现恢复。
顺势疗法药物白砷-30在减轻砷诱导的蛋白质和核酸损伤方面的功效得到证实，并推断出顺势疗法药物通过调节基因表达的作用机制。
