Le Bouil A, Cailleux A, Turcant A, Allain P
Laboratoire de Pharmacologie et de Toxicologie, Centre Hospitalier Universitaire, Angers, France.
J Anal Toxicol. 1999 Jul-Aug;23(4):257-61. doi: 10.1093/jat/23.4.257.
A method for the simultaneous measurement of monomethylarsonic acid (MMA) and dimethylarsinic acid (DMA) in human urine using liquid chromatography-tandem mass spectrometry with electrospray ionization (LC-ES-MS-MS) was developed. The multiple reaction monitoring mode (MRM) was used for quantitation. The protonated molecule ions (m/z 141.0 for MMA and m/z 139.0 for DMA) were selected as precursor ions, and the same fragment ion AsO+ (m/z 91.1) was monitored as the product ion. A two-step liquid-liquid extraction of MMA and DMA from urine provided recoveries of 92-100%. The coefficients of variation were lower than 7% for the within-day precision and lower than 11% for the between-day precision. The limit of quantitation was 25 microg/L as As for the two analytes. The assay was linear over the range of 25-800 microg/L.
建立了一种使用液相色谱-串联质谱联用电喷雾电离（LC-ES-MS-MS）同时测定人尿中一甲基胂酸（MMA）和二甲基胂酸（DMA）的方法。采用多反应监测模式（MRM）进行定量。选择质子化分子离子（MMA为m/z 141.0，DMA为m/z 139.0）作为前体离子，并监测相同的碎片离子AsO+（m/z 91.1）作为产物离子。从尿液中对MMA和DMA进行两步液-液萃取，回收率为92%-100%。日内精密度的变异系数低于7%，日间精密度的变异系数低于11%。两种分析物的定量限均为25 μg/L（以As计）。该测定法在25-800 μg/L范围内呈线性。
