Romero G A, Sampaio R N, Macedo V de O, Marsden P D
Núcleo de Medicina Tropical, Universidade de Brasília, Brasília, DF, 70919-970, Brasil.
Mem Inst Oswaldo Cruz. 1999 Jul-Aug;94(4):505-8. doi: 10.1590/s0074-02761999000400014.
Sixty eight patients with localized cutaneous leishmaniasis from an area with Leishmania (Viannia) braziliensis transmission had cultures performed with a modified Marzochís vacuum aspiratory puncture technique to establish sensitivity and contamination rate with this new method. Overall sensitivity of three aspirates was 47.1%; (CI95% 39.4; 59.4) significantly greater than the sensitivity of a single one aspirate. Fungal contamination was observed in 6/204 (2.9%) inoculated culture tubes. We recommend that this useful technique should be adopted as routine for primary isolation of L. (V.) braziliensis from localized cutaneous ulcers.
在利什曼原虫（维安尼亚）巴西利什曼原虫传播地区，对68例局限性皮肤利什曼病患者采用改良的马尔佐奇真空抽吸穿刺技术进行培养，以确定这种新方法的敏感性和污染率。三次抽吸的总体敏感性为47.1%；（95%置信区间39.4；59.4）显著高于单次抽吸的敏感性。在204个接种培养管中有6个（2.9%）观察到真菌污染。我们建议将这种有用的技术作为从局限性皮肤溃疡中初步分离巴西利什曼原虫（维安尼亚）的常规方法。
