Simonini G, Cerinic M M, Generini S, Zoppi M, Anichini M, Cesaretti C, Pignone A, Falcini F, Lotti T, Cagnoni M
Department of Pediatrics, University of Florence, Italy.
Mol Cell Biochem. 1999 Jun;196(1-2):85-91.
In 63 patients affected by Systemic Sclerosis (SSc) (limited subset: 40; diffuse subset: 23; early: 30; advanced: 33) the peroxidation product diene-conjugates (DC) and antibodies against oxidised low density lipoproteins (Ab oxLDL) were tested in serum by a spectrophotometer (absorbance 234 mn) and by a standard ELISA respectively. The data were compared with those obtained by 21 healthy subjects. DC was significantly higher in patients (73.3 +/- 37.2 microM/l; p < 0.0001) than in controls (48.4 +/- 16.7) as well as in the limited (80 +/- 48.8; p < 0.05) than in the diffuse subset (64.5 +/- 36.4); and in early (84.1 +/- 31.4; p < 0.05) than in advanced stage of the disease (67.9 +/- 42.5). The levels of Ab oxLDL were significantly higher in SSc patients (309.5 +/- 367.2 mU/ml; p < 0.0001) in all its subsets (limited: 351.9 +/- 351.1, p < 0.0001; diffuse: 207.7 +/- 316.1, p < 0.05; early: 428.9 +/- 417.1, p < 0.001; advanced: 302.7 +/- 89.9, p < 0.0001) than in controls (89.3 +/- 29.1). These antibodies levels were higher in limited subset than in diffuse (p < 0.05) and in early SSc than in advanced SSc (p < 0.05). The highest values of parameters of oxidative stress are found in the early stages, when the episodes of reperfusion after ischemic episodes (Raynaud's phenomenon) are very frequent. Moreover, the damage is higher in the early stages of SSc, with intact microvessels, than in late stages, when microvessels are very reduced in number, destroyed by the worsening of the disease. These data show that the respiratory burst deduced by the lipoperoxidation is higher in SSc than in controls, and may be an important pathogenetic factors involved in tissue changes in SSc.
在63例系统性硬化症（SSc）患者中（局限性亚型：40例；弥漫性亚型：23例；早期：30例；晚期：33例），分别用分光光度计（在234nm处吸光度）和标准酶联免疫吸附测定法（ELISA）检测血清中的过氧化产物二烯共轭物（DC）和抗氧化型低密度脂蛋白抗体（Ab oxLDL）。将这些数据与21名健康受试者的数据进行比较。患者的DC（73.3±37.2μM/L；p<0.0001）显著高于对照组（48.4±16.7），局限性亚型患者的DC（80±48.8；p<0.05）高于弥漫性亚型患者（64.5±36.4）；疾病早期患者的DC（84.1±31.4；p<0.05）高于晚期患者（67.9±42.5）。SSc患者各亚型的Ab oxLDL水平（309.5±367.2mU/ml；p<0.0001）均显著高于对照组（89.3±29.1）（局限性亚型：351.9±351.1，p<0.0001；弥漫性亚型：207.7±316.1，p<0.05；早期：428.9±417.1，p<0.001；晚期：302.7±89.9，p<0.0001）。这些抗体水平在局限性亚型中高于弥漫性亚型（p<0.05），在SSc早期高于晚期（p<0.05）。氧化应激参数的最高值出现在疾病早期，此时缺血发作（雷诺现象）后的再灌注发作非常频繁。此外，在SSc早期微血管完整时的损伤程度高于晚期，晚期微血管数量非常减少，因疾病恶化而被破坏。这些数据表明，SSc患者中由脂质过氧化推导的呼吸爆发高于对照组，可能是参与SSc组织变化的重要致病因素。
