Zhao Tangjuan, Lin Huaping, Li Nan, Shi Hongmei, Kang Weijun, Xu Xiangdong
Hebei Key Laboratory of Environment and Human Health, School of Public Health, Hebei Medical University Shijiazhuang 050017 PR China
RSC Adv. 2021 Jun 4;11(33):20063-20069. doi: 10.1039/d1ra02502c. eCollection 2021 Jun 3.
A capillary electrophoresis method with on-line inhibited chemiluminescence (CL) detection was first used to determine folic acid (FA). This method was established based on the quenching effect of FA on the CL reaction of luminol with a Ag(iii) complex in alkaline medium. The separation was conducted with a 20.0 mM sodium borate buffer containing 1.0 mmol L luminol. Under optimized conditions, FA was baseline separated and detected in less than 10 min. The limit of detection of FA was 1.3 mg L, with a linear range of 5.0-150.0 mg L ( = 0.9953). The RSD value was 2.8% for intra-day precision and 5.4% inter-day precision. The recoveries of the standard addition of tablets and human urine ranged from 90.3% to 107.5% and from 82.0 to 105.7%, respectively. The proposed method was successfully applied to determine FA contents in commercial pharmaceutical tablets and human urine samples. Results suggested that this method was simple and robust.
首次采用在线抑制化学发光（CL）检测的毛细管电泳法测定叶酸（FA）。该方法基于叶酸在碱性介质中对鲁米诺与银（iii）配合物的CL反应的猝灭作用建立。使用含有1.0 mmol/L鲁米诺的20.0 mM硼酸钠缓冲液进行分离。在优化条件下，FA在不到10分钟内实现基线分离和检测。FA的检测限为1.3 μg/L，线性范围为5.0 - 150.0 μg/L（r = 0.9953）。日内精密度的RSD值为2.8%，日间精密度为5.4%。片剂和人尿加标回收率分别为90.3%至107.5%和82.0%至105.7%。所提出的方法成功应用于测定市售药片中的FA含量和人尿样品。结果表明该方法简单且可靠。
