Hood E E, Jilka J M
Cell Biology, ProdiGene, 101 Gateway Boulevard, Suite 100, College Station, TX 77845, USA.
Curr Opin Biotechnol. 1999 Aug;10(4):382-6. doi: 10.1016/S0958-1669(99)80069-X.
Foreign protein production in transgenic plants has been successful, from the generation of transgenic plant lines to the marketing of purified proteins. Antigenic proteins from disease organisms, monoclonal antibodies raised against antigens of disease organisms, and proteins with industrial process applications have been produced and tested. For vaccines, clinical trials in humans and feeding trials in animals are in progress to demonstrate their efficacy. For industrial proteins, high expression and downstream processing efficiency are key concerns, with application and test market trials in progress.
从转基因植物系的产生到纯化蛋白的上市,在转基因植物中生产外源蛋白已取得成功。来自致病生物的抗原蛋白、针对致病生物抗原产生的单克隆抗体以及具有工业生产应用价值的蛋白均已生产并经过测试。对于疫苗而言,针对人类的临床试验和针对动物的喂养试验正在进行,以证明其有效性。对于工业用蛋白,高表达和下游加工效率是关键问题,目前正在进行应用和测试市场试验。