Thibault V, Benhamou Y, Seguret C, Bochet M, Katlama C, Bricaire F, Opolon P, Poynard T, Agut H
Laboratoire de Virologie, Service d'Hépato-Gastro-Entérologie, Service des Maladies Infectieuses, Groupe Hospitalier Pitié-Salpêtrière, 75013 Paris, France.
J Clin Microbiol. 1999 Sep;37(9):3013-6. doi: 10.1128/JCM.37.9.3013-3016.1999.
Mutations associated with hepatitis B virus (HBV) resistance to lamivudine have not been extensively addressed in human immunodeficiency virus (HIV)-HBV coinfection. We have studied the HBV polymerase sequences from nine coinfected patients who experienced HBV recurrence while under lamivudine treatment. In seven of these nine patients, Met(550), belonging to the highly conserved YMDD motif, was mutated to Val and was associated with a substitution of Met for Leu(526) in each case. In the two remaining patients, we found a Met(550)-to-Ile change that was associated in only one case with a Leu(526)-to-Met mutation. No mutation was observed in three control patients not receiving lamivudine. This study demonstrates the emergence of particular genetic profiles in HBV-HIV-coinfected patients experiencing a loss of control of HBV infection despite high doses of lamivudine.
与乙型肝炎病毒(HBV)对拉米夫定耐药相关的突变在人类免疫缺陷病毒(HIV)-HBV合并感染中尚未得到广泛研究。我们研究了9例在拉米夫定治疗期间出现HBV复发的合并感染患者的HBV聚合酶序列。在这9例患者中的7例中,属于高度保守的YMDD基序的甲硫氨酸(Met)(550)突变为缬氨酸(Val),并且在每种情况下均与亮氨酸(Leu)(526)被甲硫氨酸替代有关。在其余2例患者中,我们发现甲硫氨酸(Met)(550)变为异亮氨酸(Ile),仅在1例中与亮氨酸(Leu)(526)变为甲硫氨酸的突变有关。在3例未接受拉米夫定治疗的对照患者中未观察到突变。这项研究表明,尽管使用了高剂量拉米夫定,但在HBV-HIV合并感染且HBV感染失控的患者中出现了特定的基因谱。