Kudoh K, Shimizu J, Ishiyama A, Wada M, Takita T, Kanke Y, Innami S
Department of Nutritional Science, Faculty of Applied Bioscience, Tokyo University of Agriculture, Japan.
J Nutr Sci Vitaminol (Tokyo). 1999 Apr;45(2):173-81. doi: 10.3177/jnsv.45.173.
The study was conducted to elucidate the effects of orally administered indigestible saccharides (IDS) on immunoresponses of the intestinal tracts, especially secretion and excretion of immunoglobulin A (IgA). Male 4-week-old Sprague-Dawley rats were fed diets containing several kinds of IDS (cellulose, corn husk, glucomannan, curdlan and lactulose) at 5% for three weeks. The results indicated that the proportion of IgA-presenting lymphocytes in the cecal mucosa of the tested IDS groups increased significantly or tended to increase compared with that of the cellulose group. No significant differences among the experimental groups were observed in the CD4(+)- and CD8(+)-presenting lymphocytes and the CD4+/CD8+ ratios in the small intestine, cecum and mesenteric lymph nodes. IgA amounts in the cecal contents increased significantly in the glucomannan and curdlan groups as compared with that in the cellulose group. The inconsistent results were observed in the cecal IgA amounts of the lactulose group. Although IgA excretion into feces increased periodically in the cellulose, hardly any changes were observed in the glucomannan and curdlan groups. These results revealed that IgA secretion from cecal mucosa to contents was promoted, and its excretion to feces was decreased by the oral administration of highly fermentable IDS, respectively, while non- or low-fermentable IDS functioned adversely to IgA responses in the intestinal tract. It is suggested that the response of IgA in the intestinal immune system differs with the type of IDS ingested.
本研究旨在阐明口服难消化糖类（IDS）对肠道免疫反应的影响，尤其是免疫球蛋白A（IgA）的分泌和排泄。将4周龄雄性斯普拉格-道利大鼠喂食含5%几种IDS（纤维素、玉米皮、葡甘露聚糖、凝胶多糖和乳果糖）的饲料，持续三周。结果表明，与纤维素组相比，受试IDS组盲肠黏膜中呈现IgA的淋巴细胞比例显著增加或有增加趋势。在小肠、盲肠和肠系膜淋巴结中，实验组之间在呈现CD4(+)和CD8(+)的淋巴细胞及CD4+/CD8+比值方面未观察到显著差异。与纤维素组相比，葡甘露聚糖组和凝胶多糖组盲肠内容物中的IgA量显著增加。乳果糖组盲肠IgA量出现不一致的结果。虽然纤维素组粪便中IgA排泄量呈周期性增加，但葡甘露聚糖组和凝胶多糖组几乎未观察到任何变化。这些结果表明，口服高发酵性IDS分别促进了盲肠黏膜向内容物的IgA分泌，并减少了其向粪便的排泄，而不可发酵或低发酵性IDS对肠道IgA反应起相反作用。提示肠道免疫系统中IgA的反应因摄入的IDS类型而异。
