Sherrier D J, Prime T A, Dupree P
University of Cambridge, Department of Biochemistry, United Kingdom.
Electrophoresis. 1999 Jul;20(10):2027-35. doi: 10.1002/(SICI)1522-2683(19990701)20:10<2027::AID-ELPS2027>3.0.CO;2-A.
Remodeling of the plant cell surface occurs during the establishment of cell polarity, cellular differentiation, and organ development. This report demonstrates the existence of multiple glycosylphosphatidylinositol (GPI)-anchored proteins in the model plant Arabidopsis. Using two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), we also show that GPI-anchored proteins are a relatively abundant class of protein and that they are present at the plant plasma membrane. Furthermore, some of these proteins are released into the extracellular matrix. At least one of these is an arabinogalactan protein (AGP), a class of proteins known to be associated with cellular differentiation. Analysis of the amino acid sequences of two novel AGP-like proteins from Arabidopsis predicts that these proteins contain consensus signals for GPI-anchor addition. These findings support a model where GPI-anchored proteins are involved in the generation of specialized cell surfaces and extracellular signaling molecules.
植物细胞表面重塑发生在细胞极性建立、细胞分化和器官发育过程中。本报告证明了模式植物拟南芥中存在多种糖基磷脂酰肌醇（GPI）锚定蛋白。使用二维十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE），我们还表明GPI锚定蛋白是一类相对丰富的蛋白质，并且它们存在于植物质膜上。此外，其中一些蛋白质被释放到细胞外基质中。这些蛋白质中至少有一种是阿拉伯半乳聚糖蛋白（AGP），这是一类已知与细胞分化相关的蛋白质。对来自拟南芥的两种新型AGP样蛋白的氨基酸序列分析预测，这些蛋白质含有添加GPI锚定的共有信号。这些发现支持了一个模型，即GPI锚定蛋白参与特殊细胞表面和细胞外信号分子的产生。
