Kato T, Mizokami M, Orito E, Nakano T, Tanaka Y, Ueda R, Hirashima N, Iijima Y, Kato T, Sugauchi F, Mukaide M, Shimamatsu K, Kage M, Kojiro M
Second Department of Medicine, Nagoya City University Medical School, Nagoya, Japan.
J Hepatol. 1999 Aug;31(2):221-7. doi: 10.1016/s0168-8278(99)80217-7.
BACKGROUND/AIMS: Although a novel DNA virus, TT virus (TTV), has been isolated from a patient with cryptogenic post-transfusion hepatitis, its pathogenic role remains unclear. To elucidate its prevalence and clinical impact in patients with liver diseases, the presence of TTV DNA was assessed in patients with liver diseases and blood donors (BDs) in Japan using two primer sets, one conventional and the other new and highly sensitive.
We studied 261 samples, 72 with chronic hepatitis associated hepatitis C virus (HCV-CH), 57 with hepatocellular carcinoma associated HCV (HCV-HCC), 12 with HCC without either HCV or hepatitis B virus (NBNC-HCC), and 120 of BDs.
Using two primer sets, TTV DNA was detected in 68 (94.4%), 53 (93.0%), 12 (100%), and 98 (81.7%) HCV-CH, HCV-HCC, NBNC-HCC, and BDs, respectively. The prevalence was not significantly different between HCV-CH and HCV-HCC, or between HCV-HCC and NBNC-HCC. Comparison between patients with and without TTV revealed no significant differences in backgrounds or biochemical findings. Histopathological findings in patients with HCV-CH, and number, maximum diameter, and histological differentiation of HCC also did not demonstrate any relation to TTV infection. TTV strains can be divided into five groups using phylogenetic analysis, but no disease-specific group appears to exist.
Our data suggest that: 1) TTV is very prevalent among patients with liver diseases and even among BDs in Japan, 2) TTV infection does not impact on liver damage with HCV infection, and 3) TTV infection also does not affect the development or progression of HCC.
背景/目的：尽管一种新型DNA病毒，即TT病毒（TTV），已从一名不明原因的输血后肝炎患者中分离出来，但其致病作用仍不清楚。为了阐明其在肝病患者中的流行情况及其临床影响，我们使用两组引物（一组为传统引物，另一组为新型高灵敏度引物）对日本的肝病患者和献血者（BD）进行了TTV DNA检测。
我们研究了261份样本，其中72例为慢性丙型肝炎病毒相关性肝炎（HCV-CH），57例为丙型肝炎病毒相关性肝细胞癌（HCV-HCC），12例为既无HCV也无乙型肝炎病毒的肝细胞癌（NBNC-HCC），以及120例献血者。
使用两组引物，分别在68例（94.4%）HCV-CH、53例（93.0%）HCV-HCC、12例（100%）NBNC-HCC和98例（81.7%）献血者中检测到TTV DNA。HCV-CH和HCV-HCC之间，以及HCV-HCC和NBNC-HCC之间的流行率没有显著差异。TTV阳性和阴性患者之间在背景或生化检查结果方面没有显著差异。HCV-CH患者的组织病理学检查结果，以及HCC的数量、最大直径和组织学分化也与TTV感染没有任何关系。通过系统发育分析，TTV毒株可分为五组，但似乎不存在特定疾病组。
我们的数据表明：1）TTV在日本的肝病患者中甚至在献血者中非常普遍；2）TTV感染不影响HCV感染所致的肝损伤；3）TTV感染也不影响HCC的发生或进展。
