Hafez Mohamed M, Shaarawy Sabry M, Hassan Amr A, Salim Rabab F, Abd El Salam Fatma M, Ali Amal E
Virology and Immunology Unit, Cancer Biology Department, National Cancer Institute, Cairo University, 1st Kasr El-Aini st, Cairo, Egypt.
Virol J. 2007 Dec 6;4:135. doi: 10.1186/1743-422X-4-135.
Transfusion Transmitted virus (TTV) is a novel single-stranded DNA virus that was identified in patients with post-transfusion hepatitis of non-A-G type. Clinical significance of TTV infection was analyzed in Egyptian hepatocellular carcinoma (HCC) patients. The present study attempted to clarify these issues in Egypt, particularly in Qaluobia governorate, a country known for its high endemicity of liver disease and hepatotropic viruses.
TTV are determined in the serum of 60 samples obtained from HCC and liver cirrhosis (LC) patients and 30 healthy individuals. TTV DNA is amplified by nested-PCR with TTV-specific mixed primers derived from the conserved open reading frame 1 (ORF1) region followed by digestion with restriction enzyme. Using the enzymes HaeIII, DraI, EcoRI and PstI, we are able to distinguish between the four TTV genotypes.
The positive rate of TTV detection was 46.7%, 40% and 36.7% among HCC, LC patients and healthy individuals respectively. The more prevalence genotype was detected in the positive serum samples was genotype 1 (35.7%) in HCC patients, (50%) in LC and (63.3%) in healthy individuals, Genotype 5 (21.4%), (25.5%) and (18.2%) in HCC, LC and healthy individuals respectively.
This study indicates that TTV is commonly present in adult patients with HCC and LC as well as healthy individuals. The most prevalence TTV genotype is genotype 1. It seems that the infection neither contribute to the severity of liver disease no to the causation of HCC.
输血传播病毒（TTV）是一种新型单链DNA病毒，在非甲 - 戊型输血后肝炎患者中被发现。对埃及肝细胞癌（HCC）患者中TTV感染的临床意义进行了分析。本研究试图在埃及，特别是在肝病和嗜肝病毒高流行的盖勒尤比省澄清这些问题。
对从HCC和肝硬化（LC）患者以及30名健康个体获得的60份血清样本进行TTV检测。用源自保守开放阅读框1（ORF1）区域的TTV特异性混合引物通过巢式PCR扩增TTV DNA，然后用限制酶消化。使用HaeIII、DraI、EcoRI和PstI酶，我们能够区分四种TTV基因型。
HCC患者、LC患者和健康个体中TTV检测的阳性率分别为46.7%、40%和36.7%。在阳性血清样本中检测到的最常见基因型在HCC患者中为基因型1（35.7%），在LC患者中为（50%），在健康个体中为（63.3%）；基因型5在HCC、LC和健康个体中分别为（21.4%）、（25.5%）和（18.2%）。
本研究表明TTV在成年HCC和LC患者以及健康个体中普遍存在。最常见的TTV基因型是基因型1。似乎该感染既不加重肝病的严重程度，也不是HCC的病因。
