Ueno H, Yamada H, Tanaka I, Kaba N, Matsuura M, Okumura M, Kadosawa T, Fujinaga T
Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Biomaterials. 1999 Aug;20(15):1407-14. doi: 10.1016/s0142-9612(99)00046-0.
Chitosan is a polymeric beta(1 --> 4) glucosamine (2-amino-2-deoxy-D-glucose) and N-acetyl-D-glucosamine (2-acetamido-2-deoxy-D-glucose) which has been reported as a wound healing accelerator. In order to evaluate the efficacy of chitosan as an accelerator of wound healing, experimental open skin wounds were made on the dorsal side in three normal beagles. Cottonfiber-type chitosan (degree of acetylation = 18%) was applied for 15 days, and the process of wound healing was evaluated histologically and immunohistochemically. On day 3 postwounding, the chitosan-treated wounds showed histologically severe infiltration of polymorphonuclear (PMN) cells and an increase in effusion compared with that in the control. Granulation was more pronounced by the chitosan treatment on day 9 and 15 postwounding. Immunohistochemical typing of collagen I, III and IV showed increase of the production of type III collagen in the chitosan group. The appearance of mitotic cells occurred numerously in the control on postwounding day 3, and in the chitosan group on postwounding day 6. These results suggest chitosan to be having a function in the acceleration of infiltration of PMN cells at the early stage of wound healing, followed by the production of collagen by fibroblasts.
壳聚糖是一种聚合的β(1→4)葡糖胺（2-氨基-2-脱氧-D-葡萄糖）和N-乙酰-D-葡糖胺（2-乙酰氨基-2-脱氧-D-葡萄糖），据报道它是一种伤口愈合促进剂。为了评估壳聚糖作为伤口愈合促进剂的功效，在三只正常的比格犬背部制作了实验性开放性皮肤伤口。应用棉纤维型壳聚糖（乙酰化程度 = 18%）15天，并通过组织学和免疫组织化学方法评估伤口愈合过程。在受伤后第3天，与对照组相比，壳聚糖处理的伤口在组织学上显示多形核（PMN）细胞严重浸润且渗出增加。在受伤后第9天和第15天，壳聚糖处理使肉芽组织更明显。I、III和IV型胶原蛋白的免疫组织化学分型显示壳聚糖组中III型胶原蛋白的产生增加。有丝分裂细胞在受伤后第3天的对照组中大量出现，在受伤后第6天的壳聚糖组中大量出现。这些结果表明壳聚糖在伤口愈合早期具有促进PMN细胞浸润的功能，随后成纤维细胞产生胶原蛋白。
