Okamoto Y, Shibazaki K, Minami S, Matsuhashi A, Tanioka S, Shigemasa Y
Department of Veterinary Surgery, Faculty of Agriculture, Tottori University, Japan.
J Vet Med Sci. 1995 Oct;57(5):851-4. doi: 10.1292/jvms.57.851.
Analysis on the accelerating effects of open wound healing by chitin and chitosan were carried out in dogs. Two, square, full-thickness wounds of skin (2 x 2 cm2) were created on the each dog's both sides of dorsal midline at 0, 14, 21, and 24 days. In one dog, one wound (left side) was treated with chitin (chitin group) and the other wound (right side) was not treated (control group). In another dog, one wound (left side) was treated with chitosan (chitosan group) and the other wound (right side) was not treated (control group). At 28 days after initial wounding, each wound site including surrounding tissue was taken for macroscopic and histological observations. Reepithelialization tended to be greater in chitin and chitosan groups than in the control group. However, when the scores of reepithelialization and granulation tissue were evaluated statistically, there was no significant differences in three groups during experimental period. Number of inflammatory cells was greater statistically in level in the control group than those in chitin and chitosan groups at 28 days after wounding. Many rete ridges were observed in the control group but very few in the another groups.
对几丁质和壳聚糖促进犬开放性伤口愈合的作用进行了分析。在第0、14、21和24天，于每只犬背部中线两侧各制造两个2×2平方厘米的方形全层皮肤伤口。在一只犬身上，一个伤口（左侧）用几丁质处理（几丁质组），另一个伤口（右侧）不做处理（对照组）。在另一只犬身上，一个伤口（左侧）用壳聚糖处理（壳聚糖组），另一个伤口（右侧）不做处理（对照组）。在初次创伤后28天，取每个伤口部位包括周围组织进行大体和组织学观察。几丁质组和壳聚糖组的再上皮化倾向比对照组更明显。然而，当对再上皮化和肉芽组织的评分进行统计学评估时，在实验期间三组之间没有显著差异。在创伤后28天，对照组炎症细胞数量在统计学上高于几丁质组和壳聚糖组。在对照组中观察到许多 rete 嵴，但在其他组中很少。
