Dantas D C, Queiroz M L
Department of Physiology and Biophysical, Institute of Biology, State University of Campinas, Campinas-SP, Brazil.
Int J Immunopharmacol. 1999 Aug;21(8):499-508. doi: 10.1016/s0192-0561(99)00028-4.
In this study we investigated the effects of the treatment with Chlorella vulgaris extract (CVE) on the hematopoietic response of granulocyte-macrophage colony-forming unit (CFU-GM) of mice infected with a sublethal dose of Listeria monocytogenes (1 x 10(4) organisms/animal). CVE was given orally as 50 mg/kg/day for 5 days. In the CVE treated/infected groups L. monocytogenes was administered at the end of CVE treatment. The colony stimulating activity of the serum (CSA) was also studied in all groups. Although no effects on CFU-GM, as compared to controls, were observed in the groups receiving CVE alone, the extract produced an increase in CSA levels as compared to controls. On the other hand, the presence of the infection led to a significant reduction in the numbers of CFU-GM as observed at 48 and 72 h after the infection, in spite of the significant increase in serum CSA activity. CVE treatment of infected animals restored the numbers of CFU-GM to control levels. In the treated/ infected group the increased serum CSA was significantly higher than that observed in the only infected group. The CVE treatment (50 and 500 mg/kg) of mice infected with a dose of 3 x 10(5) bacteria/animal, which was lethal for all the non-treated controls, produced a dose-response protection which led to a 20 and 52% survival, respectively. These results demonstrated that CVE produces a significant increase in the resistance of the animals infected with L. monocytogenes, and that this protection is due, at least in part, to increased CFU-GM in the bone marrow of infected animals.
在本研究中，我们调查了用普通小球藻提取物（CVE）处理对感染亚致死剂量单核细胞增生李斯特菌（1×10⁴个菌/动物）的小鼠粒细胞 - 巨噬细胞集落形成单位（CFU - GM）造血反应的影响。CVE以50毫克/千克/天的剂量口服，持续5天。在CVE处理/感染组中，单核细胞增生李斯特菌在CVE处理结束时给药。所有组还研究了血清的集落刺激活性（CSA）。尽管单独接受CVE的组与对照组相比未观察到对CFU - GM有影响，但与对照组相比，该提取物使CSA水平升高。另一方面，感染的存在导致感染后48小时和72小时观察到的CFU - GM数量显著减少，尽管血清CSA活性显著增加。对感染动物进行CVE处理可使CFU - GM数量恢复到对照水平。在处理/感染组中，血清CSA升高显著高于仅感染组。用剂量为3×10⁵个细菌/动物感染小鼠（该剂量对所有未处理的对照组均致死），CVE处理（50和500毫克/千克）产生了剂量反应性保护，分别导致20%和52%的存活率。这些结果表明，CVE使感染单核细胞增生李斯特菌的动物抵抗力显著增强，并且这种保护至少部分归因于感染动物骨髓中CFU - GM的增加。
