Conklin D, Lofton-Day C E, Haldeman B A, Ching A, Whitmore T E, Lok S, Jaspers S
ZymoGenetics, Inc., 1201 Eastlake Avenue East, Seattle, Washington, 98102, USA.
Genomics. 1999 Aug 15;60(1):50-6. doi: 10.1006/geno.1999.5899.
A new member of the insulin gene superfamily (INSL5) was identified by searching EST databases for the presence of the conserved insulin B-chain cysteine motif. Human and murine INSL5 are both polypeptides of 135 amino acids, matching the classical signature of the insulin superfamily. Through the B- and A-chain regions, human INSL5 has 48% identity to shark relaxin, 40% identity to human relaxin, and 34% identity to human Leydig insulin-like factor. Northern blot analysis detected expression of human INSL5 in rectal, colon, and uterine tissue and of murine INSL5 only in thymic tissue. Using quantitative RT-PCR, expression of murine INSL5 was detected in the highest quantity in colon followed by thymus, and minimal expression was seen in testis. By radiation hybrid mapping and the use of surrounding markers, human INSL5 maps to chromosome 1 in the 1p31.1 to 1p22.3 region.
通过在EST数据库中搜索保守的胰岛素B链半胱氨酸基序，鉴定出胰岛素基因超家族的一个新成员（INSL5）。人和小鼠的INSL5均为135个氨基酸的多肽，符合胰岛素超家族的经典特征。通过B链和A链区域，人INSL5与鲨鱼松弛素的同源性为48%，与人类松弛素的同源性为40%，与人类睾丸间质细胞胰岛素样因子的同源性为34%。Northern印迹分析检测到人INSL5在直肠、结肠和子宫组织中的表达，而小鼠INSL5仅在胸腺组织中表达。使用定量RT-PCR，在小鼠中检测到INSL5在结肠中的表达量最高，其次是胸腺，在睾丸中的表达量最低。通过辐射杂种图谱分析并使用周围的标记物，将人INSL5定位于1号染色体的1p31.1至1p22.3区域。
